Chapter 18 Flashcards
Characteristics of life?
Cellular, metabolism, genetic material, growth and reproduction, ability to evolve
Eukaryotic diversity; types
Protists and fungi, plants, animals
Ways to classify organisms?
By similar features
Animal, vegetable, mineral (Linnaeus)
Microscopes (microorganisms)
Kingdoms: bacteria (prokaryotes), animals, plants, fungi, single celled eukaryotes
all organisms:
-have cell membrane and ribosomes
- common set of metabolic pathways like glycolysis
-replicate semiconservatively
-use DNA as genetic code
all of these are support that life has common ancestors
3 domains of life
bacteria, archaea, eukarya
bacteria
- have peptoglycan in cell wall
- coccus, bacillus, spirilium
archaea
unicellular, no mitosis (binary fission), no organelles in cytoplasm
eukarya
have nucleus, allowed evolution
how to classify organisms
- similar features
- animal/vegetable/mineral (linnaeus)
- microscopes (microorganisms)
- morphological features
how to know if organisms are related?
similar genome
gram stain is useful for
identifying species, not phylogeny
gram positive
cell wall will take up violet dye
- means cell has 5x peptidoglycan in cell wall
gram negative
appear pink from red dye
-means cell wall is under outer membrane, thin peptidoglycan layer
how is rRNA useful in genetic similarity assays
rRNA was first widely compared sequence. 5s rna is short, evolves slowly
similarity in Archaea and Eukarya
- ribosomes are not sensitive to cloramphenicol and streptomycin
- ribosomes are sensitive to diptheria toxin
variation in prokaryotes
- all tiny
- rely on diffusion for movement
- can move by flagella, twisting, gliding
- use quorum sensing
- single celled, but live in colonies
- fission, exchange genetic material
quorum sensing
signal that produces luciferase which causes bioluminescense, triggers biofilm formation
biofilm
- microorganisms in polysaccharide matrix
- difficult to kill with antibiotics
horizontal gene transfer (lateral)
1 species to the other
important: no specificity, multiple species exchange DNA
vertical gene transfer
one generation to the next
what is important about photosynthesis and evolution?
photosynthesis is common among bacteria that are not closely related, proving common ancestry and divergent evolution
what is E. Coli
proteobacteria
proteobacteria
gram negative photoautotrophs, use light reaction to metabolize sulfur
firmicutes
low GC gram positive bacteria