Chapter 12 kinematics equations

Question 1

Michaelis-Menten Plot

Answer 1

• reaction velocity (y)
  -substrate concentration (x)
  -has a hyperbola (non-allosteric enzyme)

Question 2

Michaelis-Menten equation (MM equation)

Answer 2

Vo=(Vmax) x [S] / [S] + KM

*some of the units will have a ‘ based on competitive and noncomp inhibition

Question 3

MM assumptions

Answer 3

1, Assumes ES complex
2. Assumes [E]««[S]
3. assumes a steady state: d[ES]/dt=0
4. assumes that k2 is the smallest rate constant (vo=k2[ES])

Question 4

MM constant

Answer 4

Km= (K2+K-1)/K1

Question 5

minimum rate for MM (equation)

Answer 5

vo=k2[ES]

Question 6

maximum rate for MM (equation)

Answer 6

Vmax=k2[ET]

*[ET]–> total enzyme concentration

Question 7

Km=[S] when

Answer 7

Vo = 1/2 Vmax

Question 8

Turnover number (equation)

Answer 8

Kcat=K2=Vmax/ET

*measures catalytic activity

Question 9

catalytic efficiency (equation)

Answer 9

efficiency= Kcat/KM

*measures how perfect the enzyme is / how it performs
*usually between 10^7 to 10^9 M-1 x s-1

Question 10

LB plot: slope

Answer 10

slope=kM/Vmax

Question 11

LB plot: y-int

Answer 11

y-int= 1/Vmax

Question 12

LB plot x-int

Answer 12

x-int= -1/KM

Question 13

competitive: alpha

Answer 13

alpha= Km’ / km

Question 14

non competitive: alpha

Answer 14

alpha= Vmax/ Vmax’

Question 15

Ki equation

Answer 15

ki= [I] / alpha-1