Chapter 11

Question 1

What direction does Taq Polymerase

Answer 1

Taq Polymerase always copies from 5’ -> 3’ according to the “Nascient” Strand
(the newly formed strand)

Question 1

Can Taq Polymerase bind to single stranded DNA?

Answer 1

NO! Polymerase can only bind to double stranded DNA, which is why primers are needed

Question 2

What is the difference between Endonuclease and Exonuclease?

Answer 2

ENDONUCLEASE: cuts on dsDNA

EXONUCLEASE: cuts on ends of DNA

Question 3

What is DNA dependent on?

Answer 3

Template!

DNA Polymerase
- DNA -> DNA

RNA Polymerase
- DNA -> RNA

RTase
- RNA -> DNA

Question 4

What is meant by bidirectional replication?

Answer 4

Both DNA strands are replicated simultaneously
(possible in both prokaryotic circular dsDNA and eukaryotic double helix dsDNA)

• Replication bubbles!!

Question 5

How does the spell check function work in DNA replication?

Answer 5

Polymerase detects an irregular base by its shape and stops then rocks back to allow exonuclease to come in and fix the mutation.