Chapter 10: Regulatory Strategies

Question 1

Enzymatic activity regulation

Answer 1

1. Allosteric control
2. Multiple forms of enzymes
3. Reversible covalent modification
4. Proteolytic activation
5. Controlling the amount of enzyme present

Question 2

Isozymes

Answer 2

Isozymes differ in amino acid makeup but catalyze the same reaction, typically with different Km or other responses

Allow for ”fine-tuning of metabolism”

Question 3

Enzyme that dephosphorylates proteins

Answer 3

Protein phosphatase

Question 4

Zymogens

Answer 4

Enzymes often synthesized as an inactive precursorthat gets activated via proteolytic cleavage

A.k.a. proenzymes

Question 5

Amino acids that get phosphorylated

Answer 5

Serine

Threonine

Tyrosine

Question 6

Specific activity

Answer 6

The ratio of enzyme units per ml divided by the concentration of protein in mg/ml

Specific activity values are therefore quoted as units/mg