Chapter 10- Regulation **************

Question 1

what is the most important biologically type of regulation?

Answer 1

allostery

Question 2

standard inhibition is ….

Answer 2

inhibition of michalies menten behaving enzyme by an inhibitor binding to and then changing the structure and function of the enzyme either by binding to the active site as a competitive inhibitor or to a distant site and only affecting the catalytic ability of the enzyme as a non competitive standard inhibitor or by binding to a distant site and affecting both the binding ability and the catalytic ability

Question 3

is standard inhibition wide spread in biology?

Answer 3

no

Question 4

what are the different types of regulation of enzymes?

Answer 4

allostery
covalent modification
regulatory subunits
zymogen activation

isozymes and expression (not enzyme regulation but relates to enzyme activity)

standard inhibition

Question 5

biochemical pathways have what?
regulated by what?
they usually involve what?

Answer 5

committed steps regulated by allostery usually involving feedback inhibtion

Question 6

what is feedback inhibition?

Answer 6

it inhibits or the stops the production of one of the beginning reactions to avoid making to much of one product

Question 7

what is the committed step?

Answer 7

the first step that is committed to only one product

Question 8

what does it mean to upregulate?

Answer 8

to make more of

Question 9

what does it mean to downregulate?

Answer 9

to make less of

Question 10

how do we determine what the committed step is?

Answer 10

by determining which step has AN ALLOSTERIC REGULATOr BY DETERMINING
- non Michaelis Menten kinetics
- cooperativity
- quaternary structure

Question 11

what are the three things you can do to the committed step pathways in order to be able to control your enzyme to conserve or expend energy where you need to?

Answer 11

up regulate
downregulate
feedback inhibtion

Question 12

do the interactions happen between the enzyme and the regulator directly when talking about regulation or is it just a side effect?

Answer 12

they are directly interacting with each other.

Question 13

what three things do allosteric enzymes have?

Answer 13

non Michaelis menten kinetics
cooperativity
quaternary structure

Question 14

what determines a Michaelis Menten kinetics curve?

Answer 14

rate is on the y axis
substrate concentration on the x axis
sigmoidal shape with those axis means cooperativity
which means it can be allosterically regulated

Question 15

committed step enzymes are the enzymes that are …..

Answer 15

allosterically regulated.