Chapter 10 - Classification of Microorganisms Flashcards

1
Q

Taxonomy

A

Science of classifying organisms

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2
Q

Total Number of Species

A
  • 1.7 Million Discovered

- 10-100 Million Estimated

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3
Q

The Three Domains

A
  • Archea
  • Bacteria
  • Eukarya
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4
Q

Archea

A
  • Prokaryotic
  • Found in extreme enviroments
  • No Peptidoglycan (wall)
  • Membrane has branched C chains
  • 1st Amino Acid ~ Methionine
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5
Q

Bacteria

A
  • Prokaryotic
  • All pathogens are Bacteria
  • Peptidoglycan (wall)
  • Membrane has straight C chains
  • 1st Amino Acid ~ Formylmethionine
  • Has rRNA loop and common arm of tRNA
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6
Q

Eukarya

A
  • Eukaryotic (Includes Animals, Plants, Fungi)
  • Carbohydrates (wall)
  • Membrane has straight C chains
  • 1st Amino Acid ~ Methionine
  • Has common arm of tRNA
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7
Q

Eukaryotic Species

A

Closely related organisms that breed among themselves

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8
Q

Prokaryotic Species

 1. Clone
 2. Strain
A
  • A group of cells with similar characteristics
    1. Population of cells derived from a single cell
    2. Genetically different cells within a clone or species
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9
Q

Viral Species

A

A population of viruses with similar characteristics that occupy a specific ecological niche

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10
Q

Methods of Classifying Organisms

A
  • Morphological Characteristics
  • Differential Staining
  • Biochemical Tests
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11
Q

Morphological Characteristics

A
  • Useful for identifying eukaryotes

- Classification by structure (appearance)

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12
Q

Differential Staining

A
  • Includes Gram and Acid-Fast Staining

- Classification based on cell wall composition

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13
Q

Biochemical Tests

A
  • Differentiates between closely related organisms

- Represented by a dichotomous key (if yes than… if no then…)

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14
Q

Serology

1. Antiserum

A

Study of immune responses evident in serums

1. Solution of antibodies to identify microbes

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15
Q

Serology Tests

A
  • Slide Agglunation Test (known antiserum + unknown bacterium)
  • ELISA Test (enzyme-linked immunosorbent assay)
  • Western Blotting Test (identifies antibodies in patient’s serum)
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16
Q

Phage Typing

A
  • Tests which phages a bacterium is susceptible to

- Used to find similarities among bacteria

17
Q

Genetic Tests

A
  • DNA Based Composition (% G+C)
  • DNA Fingerprint (comparison of two bacteria)
  • Nucleic Acid Hybridization
  • DNA Probe (fragment hybridization)
  • DNA Chip (identification using species specific genes)
18
Q

Nucleic Acid Hybridization

A
  • Extent of similarity between two organisms

- Combination of complimentary DNA strands