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MICROBIO > Chapter 10 > Flashcards

Chapter 10 Flashcards

1
Q

What is taxonomy?

A

classifying organism

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2
Q

phylogeny

A

study of the evolutionary history of organism

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3
Q

Domains

A

Eukarya
Bacteria
Archaea

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4
Q

Methanogens

A

produce methane

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5
Q

Halophiles

A

high salt

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6
Q

Hyperthermophiles

A

high tempertures

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7
Q

Classification

A 
Domain 
Kingdom 
Phylum 
Class 
Order 
Family 
Genus 
Species
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8
Q

What are classification based on ?

A

rRNA

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9
Q

Prokaryotic species

A

population with similar characteristics

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10
Q

Virus classificaiton

A

obligate intracellular parasites

viral species- population of viruses with similar characteristics in a similar niche

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11
Q

Classifying Bacteria

A

Morphology
Differential Staining
Biochemical test

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12
Q

Serology

A
  • antibodies are produced against bacteria

- antiserum- solution of antibodies

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13
Q

Slide agglutination

A

unknown bacteria placed on slides, antiserum added to samples, bacteria will clump when with specific antibodies

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14
Q

Negative Test = Slide agglutination

A

no clumps

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15
Q

Positive Test= Slide agglutination

A

clumps

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16
Q

Western Blotting and ELISA

A

enzyme-linked immunosorbent assay

17
Q

Phage Typing

A

which phages the bacteria is susceptible to

18
Q

DNA base composition

A

% G/C and A/T

19
Q

DNA fingerprinting

A

DNA is cut by restriction enzymes, patterns of fragments used to identify

20
Q

Polymerase Chain reaction (PCR)

A

amplifies DNA of microbe, identified using electrophoresis

MICROBIO (6 decks)

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