Chapter 10 Flashcards
Which of the following is NOT a characteristic of members of the domain Archaea? cause disease in humans may hyperthermophiles or extreme halophiles found in extreme environments carry out unusual metabolic processes lack peptidoglycan in cell walls
cause disease in humans
Which of the following is NOT included in Domain Eukarya? a human a yeast a slime mold a fern a photosynthetic bacterium
a photosynthetic bacterium
Members of the Kingdom Fungi are \_\_\_\_\_\_\_\_\_\_. usually motile prokaryotes typically found in "extreme environments" either unicellular or multicellular photosynthetic
either unicellular or multicellular
A unicellular microorganism has been isolated from an acidic sulfur-rich hot spring in Yellowstone Park. Its cell wall lacks peptidoglycan and has no nucleus or other membranous organelles. What is the most likely classification of this microorganism? gram-positive bacteria protista fungi gram-negative bacteria archaea
archaea
Which of the following is in the correct order?
domain, kingdom, phylum, class, order, family, genus, species
kingdom, domain, phylum, class, family, order, genus, species
kingdom, domain, phylum, family, order, class, genus, species
kingdom, domain, phylum, class, order, family, genus, species
domain, kingdom, phylum, family, order, class, genus, species
domain, kingdom, phylum, class, order, family, genus, species
A key difference between classification methods and identification methods is that classification methods are designed to __________.
examine an organism’s staining characteristics
determine the oxygen requirements of an organism
determine evolutionary relationships of organisms
determine if an organism is resistant to antibiotics
determine an organism’s pathogenicity
determine evolutionary relationships of organisms
Which of the following is NOT true for the slide agglutination test?
Agglutination indicates that the antibody recognizes, and thus binds, to the antigens on the cell surface.
Bacteria or other cells are mixed together with the antibody on a slide.
This test can be used to determine bacterial identity.
The test is carried out on a microscope slide.
The absence of agglutination indicates that the antibody reacted with the antigen.
The absence of agglutination indicates that the antibody reacted with the antigen.
Which of the following is NOT true of the Western blot?
After electrophoresis and transfer of proteins to a filter, specific proteins can be identified with a patient’s antibodies and an enzyme linked to anti-human serum.
It is an important test for diagnosis of both HIV infection and Lyme disease.
This technique identifies signature rRNA sequences of microbes.
After electrophoresis of serum proteins, the separated proteins are blotted onto a filter.
Proteins from a known bacterium or virus are separated by electrophoresis in this assay.
This technique identifies signature rRNA sequences of microbes.
A number of skin infections caused by MRSA (methicillin-resistant Staphylococcus aureus) were detected among members of the wrestling teams of several local high schools. Which of the following methods might demonstrate whether these infections are caused by the same bacterial strain? phage typing slide agglutination Western blotting gram stains DNA base composition (G + C profile)
phage typing
Which of these methods can identify bacteria in a sample without culturing the bacteria? flow cytometry biochemical testing fluorescent in situ hybridization (FISH) phage typing flow cytometry and fluorescent in situ hybridization (FISH)
flow cytometry and fluorescent in situ hybridization (FISH)
Two bacteria with the same percentage of guanine plus cytosine \_\_\_\_\_\_\_\_\_\_. are definitely related could be related are definitely not related must have identical genes differ from each other in the percentage of adenine plus thymine
could be related
Which of the following is/are NOT used in DNA fingerprinting? restriction enzymes DNA stain electrophoresis agarose gel antibodies
antibodies
The sequencing of rRNA __________.
plays a minor role in emerging classification schemes
is problematic because genes encoding rRNA have been extensively modified through evolution
would never involve PCR
can yield information about evolutionary relatedness
requires cells to be grown in culture before sequencing
can yield information about evolutionary relatedness
Which of the following could be a choice to track the source of hospital-acquired infections? acid-fast stain morphology determination determination of sugar-fermentation patterns Gram stain DNA fingerprinting
DNA fingerprinting
Which of the following can be used to diagnose infections by detecting specific antibodies in a patient's serum? DNA fingerprinting Western blotting PCR ELISA Western blotting and ELISA
Western blotting and ELISA
