Chapter 1 Flashcards
List examples of general purpose media
nutrient agar and broth
brain heart infusion broth
Trypticase soy agar
What are some characteristics of enriched media
may contain special growth factors
can be used to aid in the growth of fastidious organisms (those requiring extra growth factors)
may contain organic substances such as blood, serum, or hemoglobin
General purpose media _____
grow a broad spectrum of microbes
usually contain a mixture of nutrients
are usually non-synthetic
include nutrient agar and broth
Culturing pathogens that are present in low numbers is a good application for _____ media.
enriched
Media that are important in the initial stages of isolating a specific type of microorganism from samples containing many other species, such as saliva or feces, are called
______________ media.
selective
List some selective agents used in microbiological media
antibiotics
bile salts
crystal violet dye
methylene blue dye
Enriched media are useful for ______.
the growth of fastidious organisms
growing pathogens that are present in low numbers
Differential media display visible differences in ______.
Colony color
Media color changes
Formation of gas bubbles
Colony size
Selective media with bile salts can be used to isolate ______.
intestinal pathogens
True or false: Selective media cannot be differential.
False
Dyes are often used in microbiological media as differential agents because they are used to detect ______.
pH changes
Dyes are frequently used as pH indicators in _____ media.
differential
TRUE OR FALSE:
Proper isolation requires that a small number of cells be spread on the agar surface.
False
