Ch. The Rest Of 6, 7, & 8 Test Flashcards
What is the effect of enzyme concentration on the rate of enzyme action?
Low enzyme concentration= low rate of rxn
Increasing enzyme concentration= increasing rate of rxn
Adding enzyme= no increase rate of rxn
All substrates bind with enzymes
What is the effect of substrate concentration on the rate of enzyme action?
Increasing substrate concentration= increasing rate rxn
Adding more substrate= no increase rate of rxn
Max achieved rate when all enzymes bind with the substrate
What are the two types of enzyme inhibitors?
Competitive inhibitor
Non competitive inhibitor
What is the difference between competitive and noncompetitive inhibitors?
Competitive- binds to active site
Non competitive- does not bind to the active site
What are examples of inhibitors?
Toxins, poisons, pesticides, and antibiotics
What are the two processes involved in regulation of enzyme activity?
Allosteric regulation
Feedback inhibition
How does allosteric regulation work?
Activator stabilizes the active form of the enzyme
Inhibitor stabilizes the inactive form of the enzyme
What does allosteric regulation have?
Allosteric regulated enzymes
What are Allosteric regulated enzymes made from?
Polypeptide subunits
What two forms does Allosteric regulated enzymes have?
Active
Inactive
How does feedback inhibition work?
End product of a metabolic pathway shuts down the pathway
Prevents a cell from wasting chemical resources by synthesizing more product than is needed
What is metabolism?
Totality of an organisms chemical rxns
What are the two types of metabolism?
Anabolism
Catabolism
A—>B—>C—>D
What are the reactants?
What are the intermediate products?
What is the end product?
ABC
BC
D
Each step in a rxn is catabolized by what?
Catalyst
What are the two metabolic pathways?
Catabolic
Anabolic
What is the difference between a catabolic and anabolic pathway?
Catabolic- release energy by breaking down complex molecules into simpler compounds
Anabolic- consume energy to build complex molecules from simpler ones
Why do we eat foods? (Carbs, proteins, fats and lipids)
Sources of substances for biosynthesis of cellular components
Sources of ATP (uses transport of substances, mechanical works, endergonic processes)
What are the end products of the digestion of carbs, proteins, fats and lipids?
Carbs- sugars
Proteins- amino acids
Lipids- fatty acids and glycerol
What is cellular respiration?
Break down of glucose in the presence of oxygen to carbon dioxide and water releasing ATP
Where does cellular respiration occur in a eukaryotic cell?
Mitochondria
Where does cellular respiration occur in prokaryotic cells?
Cytoplasm
Effect of substrate concentration.
Enzyme concentration- limiting (constant)=3
Substrate concentration- variable (x-axis)
Effect of enzyme concentration.
Enzyme concentration- variable (x-axis)
Substrate concentration- limiting (constant)=3
Metabolic pathways.
Series of linked rxns
Begin with a specific reactant and produce an end product
For competitive and noncompetitive inhibitors, does a rxn occur?
No
Another name for rxn?
Catalysis
_____ product feeds back to _____ reactant to make a rxn active again.
Final
Initial
In feedback inhibition the enzyme is the _____.
Final product
Anabolism.
Endergonic (ATP)
Catabolism.
Exergonic ( no ATP, releases ATP)
In cellular respiration, the oxygen in water comes from _____.
The air we breathe
What is the difference between autotrophs and heterotrophs?
Autotrophs- energy from CO2
Heterotrophs- energy form organic compounds
What is the source of energy for autotrophs?
Sunlight
Redox reaction is a reaction that involves transfer of electrons between _____. In _____, a substance loses electrons, or is oxidized. In _____, a substance gains electron, or is reduced.
Reactants
Oxidation
Reduction
C6H12O6 + 6O2 —> 6CO2 + 6 H2O + ATP
what are reactants?
What is reducing agent?
What is oxidizing agent?
What is oxidized?
What is reduced?
Glucose and oxygen
Water
Oxygen
Water
Carbon dioxide
What are the two electron carriers?
NAD and FAD
What is NAD and FADs function?
Electron carriers
Can be reversible oxidized and reduced
What are the oxidized and reduced forms of nicotinamide?
Oxidized: NAD+2H, FAD+2H
Reduced: NADH+H, FADH+H
What are the three types of phosphorylation?
Substrate level
Oxidative
Photo phosphorylation
What is phosphorylation?
Add a phosphate group to ADP to make ATP
Substrate level:
Source of phosphate?
Location in eukaryotes?
Location in prokaryotes?
Organic
Cytosol/matrix
Cytosol
Oxidative level:
Source of phosphate?
Location in eukaryotes?
Location in prokaryotes?
Inorganic Pi
Cristae
Cell membrane
Photophosphorylation:
Source of phosphate?
Location in eukaryotes?
Location in prokaryotes?
Inorganic Pi
Thylakoids in chloroplasts
Thylakoids in cell membrane
Organic compound + ADP —> pyruvate + ATP
What is the donor of phosphate to make ATP from ADP?
Organic compound
In oxidative level of phosphorylation and photophosphoylation, what is the donor of phosphate? What energy is used for oxidative level phosphorylation and what energy is used for photophosphorylation?
Pi
Light energy from the sun
Parts of mitochondria and where are they?
Matrix- inside Cristae
Cristae- wavy
Outer membrane- right outside mitochondria
Intermembrane space- between Cristae and outer membrane
What are the processes used by microbes to generate ATP?
Cellular respiration
Fermentation
What process requires oxygen?
Aerobic
What is the function of oxygen?
Serves as a final electron acceptor
Final electron acceptor of aerobic respiration?
Oxygen
Final electron acceptor of anaerobic respiration?
Anything other than oxygen
Final electron acceptor of fermentation?
Anything other than oxygen
What process generates the most ATP form one molecule of glucose?
Aerobic
What process generates the least ATP from one molecule of glucose?
Fermentation
What are the four stages of cellular respiration of glucose?
Glycolysis
Pyruvate oxidation
Citric acid cycle
Electron transport chain and chemiosmosis
What is another name for glycolysis?
EMP pathway
What part of the cell does glycolysis occur in prokaryotes and eukaryotes?
Both in cytosol
How many steps is glycolysis?
10
What happens during energy investment stage of glycolysis?
2 ATP used?
What happens in conserving in glycolysis?
Makes two private acids
After completion of glycolysis, what happens to glucose?
It is broken down into two molecules of pyruvate
After completion of glycolysis, how many net ATP are produced from on molecule of glucose? By what type of phosphorylation?
2
Substrate level
After completion of glycolysis, how many NADH are produced from one molecule of glucose?
2
What does competitive bind to?
Active site
What does noncompetitive bind to?
Enzyme
What will sugar form to?
CO2
What is NAD derived from?
Niacin
What is FAD derived from?
Riboflavin
What kind of energy do NADH and FADH have?
Potential
What is substrate and oxidative level in?
Animals, plants, bacteria, archaea
What is photophosphoylation in?
Organisms that have chlorophyll
What is Pi?
Inorganic phosphate
What are the types of cellular respiration?
Aerobic and anaerobic
What is the output of glucose?
2 pyruvate + 2 ATP + 2 NADH
What is the total ATP produced in glycolysis?
4
What is the net ATP produced in glycolysis?
2
What is the final product of glycolysis?
Pyruvate
What Happens to pyruvuc acid in pyruvate oxidation?
It is converted to acetyl CoA
How many CO2 are produced from two molecules of pyruvic acid?
2
How many NADH are produced from two molecules of pyruvic acid?
2
What is another name for citric acid cycle?
Krebs cycle, TCA
What part of prokaryotic cell does citric acid cycle occur?
Cytosol
What part of eukaryotic cell does TCA occur?
Matrix of mitochondria
What is the substance that enters the Krebs cycle?
Acetyl CoA
How many steps is the citric acid cycle?
8
After completion of TCA, for two molecules of acetyl CoA, how many ATP are produced and what is the type of phosphorylation?
2
Substrate level
After completion of Krebs cycle, for two molecules of acetyl CoA, how many NADH are produced?
6
After completion of citric acid cycle, for two molecules of acetyl CoA, how many FADH are produced?
2
After completion of TCA, for two molecules of acetyl CoA, how many CO2 are produced?
4
What composes the electron transport system?
4 protein complexes embedded in the membrane
What is the main function of the ETS?
Create a proton motive force (PMF)
What complex in the ETS accepts electrons carried by NADH?
1
What complex in the ETS accepts electrons carried by FADH?
2
As the electrons are passed from one acceptor to another in the ETS, what is released?
Energy
What is the function of the energy that is released as the electrons move down the chain?
Pump protons from the matrix to the intermembrane space
What happens to the H+ concentration outside the membrane and inside the membrane?
Out- more protons
In- less protons
What is the proton motive force?
Proton gradient
What is the final electron acceptor in ETS?
Oxygen
What is chemiosmosis?
The process of ions moving through a membrane from high to low concentration based on concentration and charge
What substance is formed when H+ go back inside the membrane through ATP synthase?
ATP
What is the type of phosphorylation that occurs in the ETS?
Oxidative
In ETS, for one NADH, how many ATPs are formed?
3
In ETS, for one FADH, how many ATPs are formed?
2
Why are there more ATP generated per molecule of NADH than per molecule of FADH?
NADH has more protons than FADH
How many ATP are produced by substrate level phosphorylation?
2
How many ATP are produced by oxidative phosphorylation?
6
Which generates more ATP? Oxidative phosphorylation or substrate level phosphorylation?
Oxidative
How many total ATP are generated?
40
How many net ATP are generated?
38
What stage generates the most NADH?
Oxidative phosphorylation
What enzymes regulate cellular respiration?
Phosphofructokinase, pyruvate dehydrogenase, isocitrate dehydrogenase
Name the inhibitors of phosphofructokinase?
ATP and citrate
Name the activator of phosphofructokinase?
ATP
Name the inhibitor of the pyruvate dehydrogenase?
NADH
What are the two processes that oxidize glucose in the absence of oxygen?
Anaerobic and fermentation
What is anaerobic?
Inorganic molecules as final electron acceptor ( prokaryotes)
What is fermentation?
Inorganic molecules as final electron acceptor
What are methanogens?
Anaerobes, methane producers
What do methanogens do?
reduce CO2 to methane
Where are methanogens found?
Sewage, swamps, marine sediments, and rice patties
What is the carbon source and final electron acceptor of methanogens?
CO2
What are sulfur and sulfate reducing bacteria?
Anaerobes
What do sulfur and sulfate reducing bacteria do?
Produce hydrogen sulfate
What do sulfur and sulfate reducing bacteria do?
Produce hydrogen sulfate
Where do sulfur and sulfate reducing bacteria live?
Mud and organic materials
What are the carbon sources and final electron acceptor of sulfur and sulfate reducing bacteria?
Sulfate
Where does oxidative phosphorylation occur in the mitochondria?
Innermembrane/cristae