Ch. 6 STUDY Flashcards

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1
Q

biotechnology

A

field that involves applying tools to the genome of an organism

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2
Q

recombinant DNA

A

resulting DNA when from two or more organisms are -><- (joined)

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3
Q

genetic engineering

mod

A

when someone modifies nucleic acids, (DNA or RNA), or entire genomes

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4
Q

plasmid

A

a small circular piece of DNA often found in bacteria

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5
Q

transgenic

A

An Organism that has genetic material from another type of Organism

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6
Q

Cloning

A

Making a copy of a piece of DNA, gene, genome, or an entire individual

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7
Q

Reproductive cloning

A

Using adult cells from an adult to make a new individual

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8
Q

Therapeutic cloning

A

Using adult cells to produce various mature adult cells

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9
Q

Stem cell

A

An undifferentiated cell that can mature into many different types of cells

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10
Q

Gene therapy

A

Altering genes in order to treat or stop disease

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11
Q

Genomics

A

field that studies order of nucleotides in DNA, their structure, function, and location

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12
Q

Proteomics

A

study of proteins produced in an organism

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13
Q

functional genomics

A

focuses on the function of genes, control, interactions between the various part of the genome

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14
Q

Comparative genomics

A

Study comparing the similarities and differences between genomes of various organisms

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15
Q

ex vivo

A

out of the llving

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16
Q

in vivo

A

in the living

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17
Q

How many individuals are needed to clone

A

3, the doner( gives nuc.), the egg( gives egg), the carrier( carry’s the child)

18
Q

another word for RFLP

A

DNA fingerprint

19
Q

What are STR’s

seq of dna base pair

A

Short sequences of DNA base pairs

20
Q

How can STR’s vary from person to person

A

The sequence of times the genes repeat

21
Q

What kinds of people can RFLP’s and STR’s not tell apart

A

identical twins (same DNA)

22
Q

When a gene from an animal, plant, or human is inserted into a bacterium what vector is used

A

a plasmid

23
Q

Would a clone of something at the same age look the same

A

no, environment

24
Q

Why is embryonic stem cell therapy unethical

A

In order to obtain the stem cell you would have to kill the embryo

25
Q

What does the guide RNA do in CRISPR

A

It determines where the DNA changes will be made

26
Q

What enzyme does RNA use to attach to CRISPR

A

CAS9

27
Q

What is the problem with making transgenic people

A

It would increase their number of genes and it would make them outside of God’s image

28
Q

What does the highest number of DNA segments look like

A

Very bright in the middle

29
Q

What does the longest segment of DNA look like

A

Sort of bright and near the negative Electro end

30
Q

What does the shortest DNA segments look like

A

Very faint and near the positive electrode end

31
Q

What does the lowest number of DNA segments look like

A

Very faint and towards the positive electrode end

32
Q

What are the three steps of the PCR process

A

Denaturing, annealing, extension

33
Q

Would increasing the cycles of a PCR test create more sensitivity or less, why

A

More sensitivity because every time a cycle is done the segments are doubled

34
Q
A
35
Q

In CRISPR experiment, some DNA appears we’re it shouldn’t, what needs to be modified

A

The RNA

36
Q

In pluripotent stem cells to treat illness, there is a de-differentiation process why is there a differentiation process?

A

After turning into their cell, it separates the cell into specific jobs

37
Q

What does restriction enzyme do

A

Cuts foreign dna

38
Q

Another name for restriction enzyme

A

Restriction endonculeases

39
Q

What produces human insulin to treat diabetes

A

Transgenic bac.

40
Q

What is a pluripotent stem cell

A

A de- differentiated adult cell that is like a stem cell

41
Q

CRISPR can insert genes to produce transgenic org. What can it also do

A

Edit genes that are already present