Ch 5 Flashcards
Dna profiling
Each rung consists of a base pair, two of four varieties of nucleic acid, and are combined in pairs called nucleotides. The sequence of these base pairs constitutes the genetic coding of DNA.
contains the full complement of an individual’s DNA, called the genome, that is unvarying from cell to cell. The genome consists of approximately 3 billion base pairs, of which about 3 million actually differ from person to person.
Person-to-person differences within a particular segment of DNA sequence are referred to as alleles.
Human cells contain two types of DNA…
Nuclear and mitochondrial
Nuclear dna
contains twenty-three pairs of chromosomes in the nuclei of the cells and each parent contributes to the genetic makeup of these chromosomes.
Mitochondria dna (mtDNA)
Found outside the nucleus and is inherited solely from the mother. Mitochondria are cell structures found in all human cells and they provide about 90 percent of the energy that the body needs to function.
polymerase chain reaction (PCR) analysis
extracting DNA from a small evidence sample and then replicating it through a complex operation of repeated heating and cooling cycles and exposure to an enzyme.
a difference of a single allele can exclude someone as the donor of that evidence sample.
CODIS
FBI is supporting this effort by linking these state databases together to form a national database known as CODIS (Combined DNA Index System).
Latent finger prints, how many points
Twelve Points of Identity Indicate a Match.
Spectrography
A spectrograph has a narrow slit to admit the radiation, a prism or grating to distribute the radiation, and a system of lenses to focus the wavelength pattern on a photographic plate. The pattern is recorded photographically as a series of short lines, each line being an image of the slit formed by the radiation of one wavelength. In analysis, the evidence sample is vaporized to incandescence by flame, arc, or spark and the radiation recorded.
Chromatography
method of separating compounds to identify the components. Modern equipment in this area is capable of identifying compounds, such as illegal drug or poison specimens.
X ray crystallography
useful for the identification of any crystalline solid or compound from which a crystalline solid derivative can be made.
X ray diffraction
of use in processing very small samples, in examining samples with noncrystalline impurities, and in identifying inorganic and mineral substances.
neutron activation analysis (NAA),
can analyze samples one hundred times too small for ordinary spectrographic techniques.
NAA measurements for the presence of antimony and barium, which are common gunshot residues,
Gamma ray spectrometer
used to measure the distinctive radioactive gamma-ray emissions and thereby to identify the elements from which the rays originate.
ultrasonic cavitation
etching method that may replace chemical, electrolytic, and magnetic particle methods of restoring obliterated serial numbers on firearms and other metal objects.
trace metal detection technique (TMDT)
Distinctive “signatures” of handguns, tools, and other metal objects are now available to investigators through an innovative trace metal detection technique (TMDT), which makes the patterns of these objects visible on the skin or clothing of suspects when treated with a test solution and examined under ultraviolet light