Ch 4 - Autologous Conditioned Sera

Question 1

What is autologous conditioned sera?

Answer 1

Incubated serum samples containing increased concentrations of IL-1ra.

Used to combat/balance the ration with IL-B2 and counteract the pathway in the pathogenesis of OA

Question 2

How it autologous conditioned Sera made?

Answer 2

• 10-50ml whole blood collected with a large needle and excellend venipuncture technique
• slowly injected into a sterile, single-use chamber containing borosilicate beads
• Allowed to coagualte and incubated at 37degreesC for 6-24hr
• Leukocytes adhere to beads, induced cell signalling events leading to an increased expression and release of IL-1ra
• Centrifuges to isolate the serum
• Given as an intra-articular injection

Question 3

Literature…

Answer 3

• Management of human OA in 167 patients - no significant difference compared to placebo
• Management of OA of the knee in humans in comparison to hyaluronic acid. Significantly greater functional improvement up to 2yr post-Tx
• Horses treated with weekly series of 4 injection of middle carpal joint OA had significant clinical improvement and decreased synovial membrane hyperplasia and increased synovial fluid concentration of IL-1ra