Ch 4 - Autologous Conditioned Sera Flashcards

1
Q

What is autologous conditioned sera?

A

Incubated serum samples containing increased concentrations of IL-1ra.

Used to combat/balance the ration with IL-B2 and counteract the pathway in the pathogenesis of OA

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2
Q

How it autologous conditioned Sera made?

A
  • 10-50ml whole blood collected with a large needle and excellend venipuncture technique
  • slowly injected into a sterile, single-use chamber containing borosilicate beads
  • Allowed to coagualte and incubated at 37degreesC for 6-24hr
  • Leukocytes adhere to beads, induced cell signalling events leading to an increased expression and release of IL-1ra
  • Centrifuges to isolate the serum
  • Given as an intra-articular injection
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3
Q

Literature…

A
  • Management of human OA in 167 patients - no significant difference compared to placebo
  • Management of OA of the knee in humans in comparison to hyaluronic acid. Significantly greater functional improvement up to 2yr post-Tx
  • Horses treated with weekly series of 4 injection of middle carpal joint OA had significant clinical improvement and decreased synovial membrane hyperplasia and increased synovial fluid concentration of IL-1ra
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