Ch 26-27 Flashcards
Axenic culture
pure culture
Great plate count anomaly
discrepancy between cells observed and colonies cultivated
Viability PCR
dye enters damaged cells and prevents PCR amplification
Enrichment culture
promotes growth of target microbe, inhibits growth of others
phylotype
Organisms classified together for their shared observable traits
Forms of whole genome comparison
- average nucleotide identity
- G+C content
- Comparative Genomics
Metagenomics
sequencing all DNA from an environment
Metatranscriptomics
mRNA present in the environment is reverse transcribed and sequences=d
Metaproteonomics
identifies proteins present at the time of sampling
High frequency genes in metagenomic analysis means
they make up the core (shared portion) of the genome across organisms
Low frequency genes in metagenomic analysis means
species specific genes
Species richness
number of different species in an environment
population
group of microorganisms within a single species or other taxon in an ecosystem
guild
group of microbes defines by physiological activity
microbial community
microorganisms that share a common habitat
symbiosis
stable association of two or more organisms
facultative
alternate lifestyle possible
obligatory
host is absolutely required
mutualism
both partners benefit form each other
cooperations
nonobligatory
antagonism
one organism negatively impacts the other
syntrophy
when one species benefits from the metabolic products of another
endosymbiont
lives within the hosts cells
coevolution
evolved together because both partners cannot grow without each other