CH. 2 -- Aseptic Technique

Question 1

aseptic

Answer 1

method of handling microbes and materials in a way that minimizes contamination (a = without, sepsis = contamination)

Question 2

inoculation

Answer 2

process of transferring a microbe from one medium to the next

Question 3

inoculum

Answer 3

sample being tranferred from a medium

Question 4

inoculating loop/needle

Answer 4

made of Nichrome, a metal alloy that heats up very quickly, used to transfer an inoculum

Question 5

Bunsen burner

Answer 5

used to sterilized inoculating tools, flame should be blue, not yellow – the hottest part of the burner flame is just above the inner blue cone – metal on loop will turn bright red when sterilized

Question 6

DO NOTS::

Answer 6

1. DO NOT blow on loop to cool 2. DO NOT wave loop to cool 3. DO NOT place on bench top after sterilizing 4. DO NOT touch loop to check if cool. 5. NEVER placed caps of test tubes on counter.

Question 7

DO::

Answer 7

1. DO sterilize the loop before and after transferring specimens. 2. DO sterilize loop until metal is bright red 3. DO allow loop to cool completely before touching specimen, to avoid killing it (you can do this by pressing onto 9 o’clock spot on petri dish, until sizzle stops) 4. You can place sterilized loop in test tube rack or storage area when finished. 5. Mouth of test tubes should be sterilized before and after specimen transfer, after cap is removed.

Question 8

flocculant

Answer 8

flecks or chunks of bacterial growth scattered throughout test tube specimen

Question 9

pellicle

Answer 9

film of bacteria covering the surface of sample

Question 10

ring

Answer 10

growth only around the edge of a sample

Question 11

turbidity

Answer 11

“muddy” or “cloudy” looking sample