Centrifugation And Specimen Processing Flashcards

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1
Q

What does SOP stand for?

A

Standard Operating Procedures

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2
Q

What are the three most important samples in the labs?

3

A

Bloods
Cerebro-spinal fluid
Tissue

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3
Q

Explain sedimentation.

A

Heavier objects drift to the base of the tube

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4
Q

Explain centrifugation.

A

Use of spinning force to rapidly deposit heavier components to base of container

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5
Q

What are the four main applications of centrifugation?

A

Blood separation

Cells concentration

Cellular components separation

Chemical reactions or test procedures

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6
Q

Why is the purpose of putting blood in a centrifuge?

A

Blood is spun to separate cells from liquid plasma/serum

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7
Q

What two types of blood may be used in centrifugation?

A

Coagulated blood

Anti-coagulated blood

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8
Q

What is the liquid in coagulated blood?

A

Liquid is serum

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9
Q

What is the liquid in anti-coagulated blood?

A

Liquid is plasma

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10
Q

Why are anti-coagulants used?

A

They interfere chemically with the clotting mechanism

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11
Q

What are the three main applications of blood separation?

A

Harvesting of blood products for transfusion

Blood cell concentration for investigation of anaemia and leukaemima

Plasma or serum analysis for biological and biochemical constituents

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12
Q

What is ultracentrifugation used for?

A

Subcellular fractionation

Nucleic acid analysis

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13
Q

What is subcellular fractionation?

A

The separation of nuclei, mitochondria, lysosomes and other organelles from cells

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14
Q

What is nucleic acid analysis?

A

Separate DNA, RNA, plasmids

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