Cells Flashcards

1
Q

Which stain is used to stain acidic things blue?

A

Haematoxylin

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2
Q

Which stain is used to stain alkaline things pink?

A

Eosin

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3
Q

Which parts of the cell does haematoxylin stain blue?

A

Cell nuclei and RNA

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4
Q

Which parts of the cell does eosin stain pink?

A

Cytoplasm and. colloidal proteins

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5
Q

Which parts of the cell does eosin stain orange/red?

A

Keratin

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6
Q

Does fat stain?

A

No

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7
Q

Name some structures that alcian blue stains blue.

A
  1. Glycosaminoglycan (GAG) rich structures e.g. connective tissue
  2. Goblet cells
  3. Mast cell granules
  4. Cartilage matrix
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8
Q

Which structures does iron haematoxylin stain black?

A

Nuclei and elastic fibres

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9
Q

What colour does periodic acid schiff stain magenta?

A

Hexose sugars

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10
Q

What is the function of the nucleus?

A
  1. Brain of the cell

2. Houses DNA as chromatin within the nucleolus

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11
Q

Is the nucleus double membraned?

A

Yes

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12
Q

What is the function of the mitochondria?

A

Site of oxidative phosphorylation

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13
Q

Is the mitochondria doubled membraned?

A

Yes, the inner membrane is highly folded

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14
Q

What are the functions of the outer and inner membranes of the mitochondria?

A

Outer membrane: Lipid synthesis, fatty acid metabolism

Inner membrane: Electron transport, ATP production

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15
Q

What is the function of the matrix of the mitochondria?

A

Site of Tricarboxylic acid cycle (Kreb’s)

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16
Q

What is the function of the intramembranous space of the mitochondria?

A

Site of nucleotide phosphorylation (ADP to ATP)

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17
Q

What is the function of the RER?

A

Site of protein synthesis

18
Q

What is the function of the SER?

A
  1. Site of membrane lipid synthesis

2. Processes and stores synthesised proteins

19
Q

What is the function of the Golgi apparatus?

A

Processes and modifies macromolecules

20
Q

Name the 3 parts of the Golgi apparatus.

A

Cis golgi - protein phosphorylation
Medial golgi - adds sugars to products
Trans golgi - proteolysis of peptides into active forms

21
Q

What is the function of vesicles?

A

Transport and store material

22
Q

Which vesicles contain digestive enzymes?

23
Q

Which process are peroxisomes used for?

A

Beta oxidation. Fatty acids are broken down into carbon fragments used for generating ATP

24
Q

What is the function of the cytoskeleton?

A

Brace internal structure of cell. Maintains shape and internal organisation

25
What are microfilaments made of?
Actin
26
What are microtubules made of?
Tubulin
27
Which storage molecule has an orange-brown pigment and is common in the heart and liver?
Lipofuscin
28
Where is lipid stored?
Adipocytes and liver
29
Where is glycogen stored?
Liver and muscles
30
What is the phospholipid bilayer of cell membranes made up of?
Glycolipids: Communication Glycoproteins: Cell to cell recognition + act as receptors Cholesterol: Maintains fluidity in membrane
31
What is the function of the cell surface membrane?
1. Acts as a selective barrier to the passage of molecules. | 2. Acts as a barrier to the outside environment
32
What is the function of an adherens junction?
Joins actin bundles in one cell to another cell, keeping cells together
33
How do intermediate filaments enable cell to cell adhesion?
Via desmosomes
34
What do hemidesmosomes do?
Anchor intermediate filaments in a cell to the basal lamina
35
Which energetic process engulfs molecules into a cell?
Endocytosis
36
Which cells implement phagocytosis?
Neutrophils and macrophages
37
How does exocytosis work?
Vesicles from the Golgi apparatus fuse with the cell surface membrane. Expulsion of waste/enzymes/hormones.
38
What is facilitated diffusion?
Movement of solutes from a region of high concentration to a region of low concentration through protein channels.
39
What is passive diffusion?
Movement of a substance from a region of high concentration to a region of low concentration, down the concentration gradient.
40
What is active transport?
Movement of solutes from a region of low concentration to a region of high concentration against the concentration gradient. ATP is required.
41
What do tyrosine kinase receptors do?
Transfers a phosphate group from ATP to a protein in the cell. Acts as an on/off switch.