Cells Flashcards
Which stain is used to stain acidic things blue?
Haematoxylin
Which stain is used to stain alkaline things pink?
Eosin
Which parts of the cell does haematoxylin stain blue?
Cell nuclei and RNA
Which parts of the cell does eosin stain pink?
Cytoplasm and. colloidal proteins
Which parts of the cell does eosin stain orange/red?
Keratin
Does fat stain?
No
Name some structures that alcian blue stains blue.
- Glycosaminoglycan (GAG) rich structures e.g. connective tissue
- Goblet cells
- Mast cell granules
- Cartilage matrix
Which structures does iron haematoxylin stain black?
Nuclei and elastic fibres
What colour does periodic acid schiff stain magenta?
Hexose sugars
What is the function of the nucleus?
- Brain of the cell
2. Houses DNA as chromatin within the nucleolus
Is the nucleus double membraned?
Yes
What is the function of the mitochondria?
Site of oxidative phosphorylation
Is the mitochondria doubled membraned?
Yes, the inner membrane is highly folded
What are the functions of the outer and inner membranes of the mitochondria?
Outer membrane: Lipid synthesis, fatty acid metabolism
Inner membrane: Electron transport, ATP production
What is the function of the matrix of the mitochondria?
Site of Tricarboxylic acid cycle (Kreb’s)
What is the function of the intramembranous space of the mitochondria?
Site of nucleotide phosphorylation (ADP to ATP)
What is the function of the RER?
Site of protein synthesis
What is the function of the SER?
- Site of membrane lipid synthesis
2. Processes and stores synthesised proteins
What is the function of the Golgi apparatus?
Processes and modifies macromolecules
Name the 3 parts of the Golgi apparatus.
Cis golgi - protein phosphorylation
Medial golgi - adds sugars to products
Trans golgi - proteolysis of peptides into active forms
What is the function of vesicles?
Transport and store material
Which vesicles contain digestive enzymes?
Lysosomes
Which process are peroxisomes used for?
Beta oxidation. Fatty acids are broken down into carbon fragments used for generating ATP
What is the function of the cytoskeleton?
Brace internal structure of cell. Maintains shape and internal organisation