CELLS Flashcards
What’s a chimera?
having parts of different origins
e.g. a eukaryotic cell
What’s an endosymbiont?
organisms forming symbiotic relationships with another cell/organism
2 types of endosymbiont
intracellular
extracellular
what’s a plastid?
membrane-bound organelle
How are phylogenetic trees formed?
inferred from nucleotide/ amino acid sequence data
most common phylogenetic marker
small sub-unit ribosomal RNA (SSUrRNA)
ARCHEZOA hypothesis
eukaryogenesis involving exogenous origins of mitochondrion via phagocytosis of an alphaproteobacterium to form mitochondrion
remnant genome of mitochondria
*what does it encode
rRNA and protein-coding genes
2 rRNA’s (12S and 16S)
22 tRNA’s
13 essential genes
*encodes sub-units for oxidative phosphorylation enzyme complexes
origins of ER
Endogenous
gram-negative bacteria
don’t retain crystal-violet stain
- double membrane systems
chloroplast exogenous origin
cyano bacteria
plasma membrane functions
- enclose cell content/ separate from environment
- maintain concentrations of cell substances
- communication w environment/other cells
- barrier
- cell growth/ shape change/ movement/ division
cytosol function
protein synthesis/ metabolic pathways
endoplasmic reticulum
lipid synthesis/ protein synthesis
golgi apparatus function
modification/sorting/packaging of proteins/ lipids
endosome function
sorting of endocytosed material
plasma membrane sub-unit
* polarity of parts
phospholipid
- hydrophilic phosphate head
hydrophobic fatty acid tails
* amphiphilic!
cholesterol effect on PM
decreases membrane permeability to small/ water-soluble molecules
prevent crystallization
4 phospholipids in plasma membrane
phosphatidylcholine, phosphatidylethanolamine, phosphatidylserine, and sphingomyelin
function of glycolipid asymmetry
extracellular to intracellular signal conversion
charge differences
binding sites
live and dead cell distuinguishment
sugar group addition
self-association of glycolipids
H-bonds from sugars / Van der Waals between hydrocarbon chains
oligosaccharides charge and function
net negative charge
alters electric field and ion concentrations
lectins
carb binding proteins
bind to sugar groups on other glycolipids/ glycoproteins
how is the PM fluid
rapid lateral diffusion
flexible hydrocarbon chains
flippases catalyzing movement
cis-double bonds create kinks in fatty acid tail/ shorter tail lengths
temp effect on cis bonds
more cis bonds as temp drops
types of membrane proteins
integral
peripheral
lipid attached
when are integral proteins released?
when detergents dissolve PM
where are lipid-attached proteins made?
ER
prior to cleaving and GPI anchor added via vesicle
when are peripheral proteins released?
when protein-protein interaction disrupting agents arrive
functions of membrane proteins
transporters
linkers
receptors
catalysis
how are transmembrane domains identified?
bioinformatic analysis ‘in silico’
glycocalyx components
glycoproteins/ glycolipids
glycocalyx function
protect cell chemically, physically, biologically
adhesion
recognition
storage
affect health and disease
glycoprotein links
N-linked
O-linked
proteoglycans
N-glycans
asparagine-linked
O-linked
serine/threonine-linked
proteoglycans
glycoproteins w GAGs
polysaccharide chains covalently linked to a protein core> GPI anchor
GAG
glycosaminoglycans
ruthenium red
stains carbohydrate layer of glycocalyx
detergents
small, amphiphilic molecules of variable structure
(more soluble than lipids)
detergent behaviour
aggregate to form micelles/ rapidly diffuse in/out
affected by temp, pH and salt conc
displace lipid molecules
affect crystallization/purification
tpes of membrane diffusion
rotational
lateral
how are PM diffusion rates measured
FRAP
fluorescence recovery after photobleaching
FRAP process
- mark membrane protein w fluorescent group
- fluoresecnce group bleached w laser beam
- time for diffusion measured and diffusion coefficient measured
disadvantages of FRAP
can’t follow individual protein molecules
cortical cytoskeleton
spectrin meshwork maintaining integrity and shape of PM
*anaemia doesn’t have
cell cortex
actin filaments attached to PM
cell cortex functions
cell movement
endocytosis
filopedia production
restricts free diffusion of proteins
types of active transport
coupled
ATP-driven
light/redox driven
membrane-bending protein function
deforms bilayers
(dynamic control of membrane shape)
membrane-bending protein mechanisms
insertion of hydrophobic protein domains/ lipid anchors
rigid scaffold formation
clustering of specific membrane lipids
selectivity filter
narrowest region of gated ion channel, limiting rate of passage
types of gated ion channels
voltage-gated
ligand-gated extracellular
ligand-gated intracellular
mechanically gated