Cell Structure and Organisation Flashcards
List the organelles in an animal cell.
Golgi body, Smooth endoplasmic reticulum, rough endoplasmic reticulum, centrioles, mitochondria, lysozyme, nucleus, ribosomes, cell membrane and cytoplasm
List the organelles of a plant cell.
Plasmodesmata, chloroplasts, cytoplasm, golgi body, mitochondria, RER, nucleus, ribosomes, SER and vacuole
How to workout total magnification?
eyepiece x objective
Why must the sample be thin?
To allow light to pass through
Why must the sample be stained?
To see the organelles clearer and more visible
Why might staining be a disadvantage?
it can be toxic and harm/kill organisms
State the structures of a nucleus.
nuclear envelope, nucleoplasm, chromatin and nucleolus.
Give the function of each structure in a nucleus (there are 4)
Nuclear envelope: double membrane with pores to allow transport of mRNA and ribosomes out of nucleus to cytoplasm
Nucleoplasm: cytoplasm-like material within nucleus containing chromatin.
Chromatin: made up of coils of DNA bound to histone protein. during cell division, chromatin condenses forming visible chromosomes.
Nucleolus: one or more spherical bodies which synthesise rRNA
What is the function of mitochondria?
to release energy in the form of ATP during aerobic respiration
describe the structure of mitochondria.
they have a double membrane with a narrow fluid-filled intermembrane space. the inner membrane is folded inwards to form extensions called cristae which increase the surface area for ATP synthesis to occur. there is an organic matrix containing many chemical compounds like lipids, proteins, small 70s ribosomes and a small circle of DNA to allow self-replication in response to energy needs of the cell
Where do the stages of aerobic respiration occur in the mitochondria?
in the matrix and on inner membrane
where are large numbers of mitochondria usually found in the body?
liver and muscles (break down toxins and contraction)
What are the 2 sizes of ribosomes and which belongs to eukaryotes/prokaryotes?
80s(eukaryotes) and 70s(prokaryotes)
How is a ribosome structured?
a small subunit on a large subunit made of rRNA and protein.
How does protein synthesis occur?
in translation, mRNA fits in the groove between the 2 subunits and provides the code for a sequence of amino acids.
where are ribosomes found in a cell?
free in cytoplasm or associated with RER
What is the function and structure of RER?
its an internal system of flattened membranous sacs/cisternae which are continuous with the nuclear membrane and linked to the golgi body to help transport substances. its function is protein synthesis and transport of proteins so its present in large numbers to synthesise large quantities.
What is the function and structure of SER?
its similar in structure to RER but has no ribosomes. its involved in synthesis and transport of lipids and cells which store large quantities of carbohydrates, protein or fat, have loads of SER
Give the function and structure of the golgi body.
made up of interconnected flattened membranous sacs, and vesicles containing polypeptides pinch off from RER and fuse with the Golgi body. proteins are modified and packaged into vesicles and at the other end of the GB, vesicles containing the modified proteins bud off. they modify and package proteins into secretory vesicles for secretion from the cell, they produce glycoproteins, form lysosomes, transport and store lipids and secrete carbohydrates for formation of plant cell walls.
Give function and structure of lysosomes.
single membrane-bound vesicles that contain hydrolytic enzymes and are produced by the golgi body. they isolate potentially harmful digestive enzymes from the remainder of the cell and release hydrolytic enzymes when the cell needs to break down worn-out organelles. they also digest material that has been taken in the cell, for example, lysosomes fuse with the vesicle made when a white blood cell engulfs bacteria by phagocytosis and their enzymes digest the bacteria.
Link the function of the organelles.
DNA in the nucleus contains genetic code to make proteins/mRNA
nuclear pores in the nuclear envelope allow mRNA to leave nucleus and attach to ribosomes in RER or cytoplasm
mRNA transported through RER
protein synthesis occurs on ribosomes
vesicles pinched off from RER are transported to Golgi body
vesicles fuse with golgi membrane and contents are shed into golgi sacs
proteins formed into more complex molecules
vesicles containing modified proteins bud off at the end of golgi body
vesicles fuse with cell membranes
proteins are secreted - by exocytosis
lysosomes may be produced - digestive enzymes
How are phospholipid molecules arranged on the membrane of cells?
as a bilayer
fatty acid tails of both layers point towards inside of the membrane
hydrophilic heads point outwards, interacting with the tissue fluid/blood plasma