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BIOSCI 107 Part One > Cell Structure and Function - Introduction > Flashcards

Cell Structure and Function - Introduction Flashcards

1
Q

What is a Prokaryotic cell?

A

are cells that do not have a true nucleus or membrane-bound organelles

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2
Q

What is a Eukaryotic cell?

A

cells that have a nucleus enclosed within membranes (humans)

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3
Q

What do Eukaryotic cells and prokaryotic cells have in common?

A

The plasma membrane, cytosol, DNA, RNA,

protein and ribosomes

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4
Q

What are the main differences between eukaryotic and prokaryotic cells?

A

Eukaryotic cells are much larger and have a membrane bound nucleus while prokaryotic cells are much smaller and their nucleus is not membrane-bound

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5
Q

What is the cytoplasm?

A

is everything inside the plasma membrane including the organelles,
but not including the nucleus

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6
Q

What is cytosol?

A

The fluid portion of the cytoplasm

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7
Q

What does cytosol consist of?

A

water plus dissolved and suspended substances (eg. ions, ATP, proteins, lipids)

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8
Q

What organelles make up the endomembrane system?

A

Nucleus
Endoplasmic Reticulum (smooth and rough)
Golgi apparatus
Lysosomes

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9
Q

What is the endomembrane system?

A

A network of membranes inside and around a eukaryotic cell organelles
Work together to modify, package, and transport lipids and proteins (ie shared membrane space)

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10
Q

Order of the sizes of microvilli, cilia and flagellum

A

Microvilli < Cilia < Flagellum

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11
Q

What is the function of the plasma membrane?

A

is a selectively permeable barrier controlling
the passage of substances in and out of the cell (acts as a physical barrier separating the inside and outside of the cell)

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12
Q

What is the plasma membrane made of?

A

it is made of a double layer of phospholipids with embedded proteins

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13
Q

How does the fat portion of a phospholipid react with water?

A

It is hydrophobic so it repels water

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14
Q

How does the phosphate (head) portion of a phospholipid react with water?

A

It is hydrophilic so it will face the water

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15
Q

What does a phospholipid consist of?

A

a hydrophilic polar head (phosphate) and a hydrophobic lipid tails (fatty acids) which are arranged as a double layer around cytoplasm, tail to tail

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16
Q

What is a feature of plasma membrane proteins? (Not the 6 things they are involved in)

A

An often amphipathic protein that is associated with the plasma membrane.
Mediate movement of hydrophilic substances, allow cell-to-cell identification, adhesion between cells, attach to other structures and facilitate intercellular communication.

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17
Q

How do plasma membrane proteins react with water?

A

They are often amphipathic so they have both hydrophilic and hydrophobic regions. This is to allow molecules to pass through the plasma membrane

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18
Q

What are integral proteins

A

proteins that are embedded (partially or fully) into the membrane

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19
Q

What are transmembrane proteins?

A

proteins that fully span the entire membrane, contact extracellular
and cytoplasmic areas

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20
Q

What are Peripheral membrane proteins?

A

proteins that are associated with the

membrane, but not actually embedded in it

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21
Q

What are the functions of the plasma membrane proteins?

A 
Transport
enzymatic activity
signal transduction
cell-cell recognition
intercellular joining 
 attachment to the cytoskeleton and extracellular matrix (ECM)
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22
Q

Example of transport function of the plasma membrane

A

channels or transporters may be

general or selective, gated or not

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23
Q

Example of enzymatic activity function of the plasma membrane

A

Carry out a chemical reaction,

may or may not be a part of a team of enzymes

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24
Q

Example of signal transduction function of the plasma membrane

A

External signalling molecule causing transduction of information to the inside of
the cell

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25
Example of cell-cell recognition function of the plasma membrane
Use of glycoproteins (carbohydrate + protein) as molecular signatures of the extracellular side of the cell
26
Example of intercellular joining function of the plasma membrane
gap and tight junctions
27
Example of attachment to the cytoskeleton and extracellular matrix (ECM) function of the plasma membrane. hint = FIBRONECTIN
fibronectin mediates contact between cell surface integrins and ECM (eg. collagen). can facilitate movement
28
What is the fluid mosaic model?
The model used to describe proteins and other structure moving freely in a fluid bilayer of phospholipids.
29
What is the nucleus?
The largest distinct structure inside the cell
30
What is the structure of the nucleus
It is enclosed by a double lipid bilayer called the nuclear envelope, continuous with rough ER and has entry and exit through nuclear pores. Has the nucleolus in the centre
31
What is the function of the nucleus?
* to house/protect DNA in eukaryotic cells * Make RNA and assemble ribosomes * pores regulate the movement of substances (eg protein and mRNA) in and out * Molecule segregation to allow temporal and spatial control of cell function
32
What is the nucleolus?
Distinct structure present in the nucleus of eukaryotic cells
33
What is the function of the nucleolus?
rRNA production | Assembly of the small and large ribosomal subunits
34
What is the nuclear envelope?
Lipid bi-layer enclosing the nucleus | Continuous with the rough ER
35
What is the function of the nuclear pore and where are they?
Structures in the nuclear envelope | Allow passage of certain materials between the cell nucleus and cytoplasm
36
What is Deoxyribonucleic Acid (DNA)?
Nucleic acid that encodes our phenotype
37
What is histone?
Protein molecule which binds with 7 others to form a nucleosome
38
What is nucleosome?
Bead-like structure in chromatin | Composed of a short length of DNA wrapped twice around core of histone proteins
39
What is chromatin?
'spaghetti like' structure | strings of nucleosomes that are wound together to from fibres
40
What is a chromosome?
Bundles of tightly coiled DNA located within the nucleus of almost every cell in our body
41
What is a gene?
a DNA segment that contributes to phenotype/function
42
How many chromosomes are in a human cell?
Humans are diploid so have 2N chromosomes = 46 chromosomes (23 pairs), one from each parent 22 Autosomes and 2 sex chromosomes
43
What is a ribosome and its function?
The organelle that is the site of protein production/synthesis
44
Where are ribosomes found in the cell??
Free in the cytoplasm - making proteins to be used in the cytosol (non-endomembrane destinations) OR Attached to the rough ER making non-cytosolic proteins/endomembrane
45
How are ribosomes made?
Subunits are assembled in the nucleolus | Leave through nuclear pores (large and small subunit)
46
What is the endoplasmic reticulum?
An extensive network of tubes and tubules | Stretch out from the nuclear membrane
47
What is the function of the rough ER?
Major Function is production of: Secreted proteins Membrane proteins Organelle Proteins Proteins enter lumen within the rough ER for folding The rough ER membrane surrounds the protein to form transport vesicles which are destined for the Golgi
48
What is the structure of the RER?
A membrane that is continuous with the nuclear envelope | Dotted with attached ribosomes
49
What is the smooth ER?
An extension of the rough ER
50
What is the function of the SER
Is very cell/tissue type specific Major function: Housing unit for proteins and enzymes Synthesise lipids (including steroids and phospholipids) Storage of cell-specific proteins, not all cells make proteins
51
What is the main difference between SER and RER
Smooth ER doesn't have ribosomes (does not make proteins)
52
The function of SER in liver
Houses enzymes for detoxification and glucose release
53
The function of SER in muscles
Stores calcium ions which are used for contraction
54
What is the sarcoplasmic reticulum?
SR is a form of smooth muscle ER Found in skeletal muscle Function is to regulate Ca2+ storage and release homeostasis during and after muscle contraction
55
What is the cis face of the Golgi apparatus?
The receiving side
56
What is the trans face of the golgi apparatus?
The shipping side
57
What is the Golgi apparatus made of?
3 to 20 flattened membranous sacs called cisternae | Stacked on top of one another ( like 'pita bread')
58
What is the function of the Golgi apparatus?
Modify, sort, package and transport proteins received from the rough ER using enzymes in each cisternae Formation of: - Secretory vesicles (proteins for exocytosis) - Membrane vesicles (PM molecules) - Transport vesicles (molecules to lysosome)
59
What are lysosomes?
Vesicles formed from the Golgi membrane Contain powerful digestive enzymes Have membrane proteins that pump H+ in order to maintain an acidic pH The rest of the cell is protected by a membrane
60
What is the function of lysosomes?
Main function is digestion of substances that enter the cell: - Cell components (eg organelles, autophagy) - Entire cells (autolysis)
61
What is Gaucher metabolic disorder?
Occurs when a particular lipid is poorly degraded Results in a severe phenotype in humans (Failure of a single lysosome enzyme can cause sever disease as digestion by lysosomes is crucial)
62
What is the function of mitochondria?
Generate ATP through cellular respiration
63
What are mitochondria made of?
Outer mitochondrial membrane Inner mitochondrial membrane (with folds called cristae) Fluid filled interior cavity (called mitochondrial matrix)
64
What is cristae?
The inner mitochondrial membrane
65
What is the mitochondrial matrix?
The fluid filler filled interior cavity
66
What is the cytoskeleton?
Structural support system of the cell Contains fibres/filaments that help maintain the size, shape and integrity of the cell Acts as scaffolding across the cell Involved in intracellular transportation and cell movement
67
What are the microfilaments in the cytoskeleton made of?
Comprised of actin molecules assemble in two long chains twisted around each other Assembled and disassembled as required - DYNAMIC
68
What is the function of microfilaments?
Bear tension and weight by anchoring the cytoskeleton to the plasma membrane proteins Promote motility if required (eg Macrophage)
69
Where are microfilaments found?
Found around the periphery and lining of the interior of cell
70
What are the intermediate filaments in the cytoskeleton made of?
Comprised of a diverse range of materials Eg Keratin | Found in cytoplasm of the cell
71
What is the function of the intermediate filaments of the cytoskeleton?
Bear tension and weight throughout the cell Eg during cell anchoring Act as a scaffold for cellular organelles Eg the nucleus
72
What are the microtubules of the cytoskeleton made of?
Tubular structure Comprise of Lublin dimers (alpha and beta) coiled to from a tube Extends from centriole into cytoplasm/nucleus
73
What is the function of the microtubules of the cytoskeleton?
Support cell shape and size Guide movement of organelles (Eg vesicles from Golgi to membrane) Chromosome organisation - cell division Support and movement of cilia/flagella (sperm tail)
74
Diameter of microfilament
7nm
75
Diameter of intermediate filament
8-12nm
76
Diameter of micro-tubule
25nm with the central lumen of 15nm diameter

BIOSCI 107 Part One (12 decks)

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