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Cell Bio - Lucas > Cell Signalling > Flashcards

Cell Signalling Flashcards

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Biology 101 flashcards
1
Q

LM

A

Light Microscopy

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2
Q

TEM

A

Transmission Electron Micrograph

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3
Q

SEM

A

Scanning Electron Micrograph

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4
Q

PCR

A

Polymerase Chain Reaction

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5
Q

Petri

A

Example of culture disk. Used for in vitro experiments. Named after bacteriologist Julius Richard Petri.

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6
Q

Ligand

A

A molecule or atom / a signalling molecule.

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7
Q

Ex Vivo

A

isolated tissues (ex = part) (vivo = organism)

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8
Q

In Vivo

A

organisms (in = whole) (vivo = organism)

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9
Q

Control

A

ensure validity of experiment

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10
Q

In Vitro

A

isolated cells (in = whole) (vitro = cells). Are cell lines an example?

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11
Q

GCaMP5G

A

Fluorescent calcium indicator that can be genetically inserted into the brain of a Zebra fish to provide a readout of the calcium level in a zebra fish brain. Genetically inserted under the control of a promoter that is expressed in neurons.

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12
Q

Positive Control

A

Something you are confident will result in the effects

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13
Q

Negative Control

A

Something you are confident won’t result in the effects

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14
Q

Autocrine

A

A type of signalling where the cell both produces and receives the molecule.

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15
Q

Variables

A

determine what is measured and what is manipulated

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16
Q

Dependent Variable

A

what you measure

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17
Q

Organ Bath

A

A traditional experiment that is commonly used to investigate the physiology and pharmocology of ex vivo tissue preparations. A technique used for Ex vivo experiments. (find my iPhone). (Scrub a dub dub).

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18
Q

Cell Lines

A

a cell culture developed from a single cell adn therefore consisting of cells with uniform genetic makeup

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19
Q

Hypothesis

A

driver of the choice in an experiment

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20
Q

Functional

A

The technique itself measures the behaviour of a system

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21
Q

Microscopy

A

the technical field of using mciroscopes to view samples and objects that can not be seen w the naked eye

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22
Q

Engineered

A

Specifically genetically designed to allow for more control during study

23
Q

Culture Disks

A

A container where cell cultures (cell lines) are grown

24
Q

Radioisotopes

A

used as tracers using their radioactivity for detection

25
Q

Culture Flasks

A

A container where cell cultures (cell lines) are grown

26
Q

Light Microscopy (LM)

A

uses visible light to detect and magnify very small objects

27
Q

Multiwell plates

A

A container where cell cultures (cell lines) are grown

28
Q

dependent variable

A

what you measure

29
Q

Electrophysiology

A

The field of research studying current or voltage charges across a cell membrane

30
Q

Independent Variable

A

what you change

31
Q

Imaging technologies

A

The application of materials to create images

32
Q

Molecular technologies

A

lab techniques to study or modify DNA, RNA, or proteins

33
Q

Polymerase Chain Reaction (PCR)

A

used to detect genetic material from a specific organism, such as a virus

34
Q

Subcellular fractionation

A

cell organelles are disintegrated into their integral parts (organelles) and then separated using centrifugation

35
Q

Scanning Electron Micrograph

A

Projects and scans a focused stream of electrons over a surface to create an image

36
Q

Transmission Electron Micrograph

A

used to view thin specimens, through which electrons can pass generating a projection image

37
Q

Ligand

A

A signalling molecule

38
Q

Lupus

A

A chronic autoimmune disease

39
Q

Autoimmune

A

The immune system (usually the system that fights infections) attacks healthy tissues instead.

40
Q

Glaucoma

A

A group of eye diseases that can cause vision loss and blindness by damaging a nerve in the back of your eye called the optic nerve.

41
Q

GERD

A

gastroesophageal Reflux Disease

42
Q

Gastroesophageal Reflux Disease

A

stomach acid repeatedly flows back into the esophagus.

43
Q

Hypertension

A

High Blood Pressure

44
Q

This reading would be high blood pressure.

A

130 / 80

45
Q

This reading would be normal blood pressure

A

120 / 80

46
Q

Cholera

A

an acute diarrhoeal disease caused by ingestion of food or water contaminated with the bacterium Vibrio cholerae.

47
Q

Rheumatoid arthritis

A

A chronic autoimmune disease causing joint inflammation, degradation, and deformity.

48
Q

Crohn’s / Colitis

A

Chronic disease. A type of IBD that causes inflammation of the gastrointestinal tract.

49
Q

IBD

A

Inflammatory bowel disease

50
Q

Histamine

A

A compound that is released by cells in response to injury and in allergic and inflammatory reactions.

51
Q

Three Types of IBD

A

All chronic. Crohn’s disease / Colitis. Microscopic colitis. Ulcerative colitis.

52
Q

Crohn’s disease

A

Causes pain and swelling in the digestive tract.

53
Q

Ulcerative colitis

A

Causes swelling and sores (ulcers) in the large intestine (colon and rectum).

54
Q

Microscopic Colitis

A

Causes intestinal inflammation that’s only detectable with a microscope.

Cell Bio - Lucas (5 decks)

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