Cell Biology Lab Flashcards
What is a P-100
A micropipetter
When do you reject Ho
When the P value is less than .05
What is a P value
In statistics, the P value tells you the likelihood that a random sample selected is representative of the population. The higher the P value, the higher probability that the sample is representative of the population.
If you have 250mg of ink mixed with 500mg of water, what is the RATIO of ink to water?
1:2
What is a normal distribution?
A normal distribution is a bell shaped curve that is symmetric around its mean, and is measured in units of standard deviation.
What test in R do you run if you have a normal distribution?
Shapiro test
What test in R do you run if the data is not normally distributed?
Wilcox test
What wavelength is the spectrophotometer set at in labs 1-3
600nm
What does it mean to reject Ho?
The data collected is not statistically significant to accept the null hypothesis.
How wide are the small squares in a hemocytometer?
1mm squared
What is Trypan Blue used for?
To identify dead cells
How does Trypan Blue enter the cell
Through ruptures in the cell membrane
What is the readout of lab 4?
Percentage of budding yeast cells
What color cells do you count in lab 4
White
What enzyme generates ethanol?
Alcohol dehydrogenase
How does ethanol affect a yeast cell?
It is highly toxic
What happened to the yeast cells as the concentration of ethanol rose?
They died at a higher rate
What time points were observed in lab 4 on yeast cells?
4hr, 4.5hr, 5hr
What regenerates NAD+?
3-glycol dehydrogenase
How are enzymes named?
By their function
What is the purpose of SDS-Page?
To sort proteins by size
Why do you use CBB
To determine if you have enough protein to work with
How are the proteins moved through the gel
An electric field
What speed do you centrifuge at
Max rpm (15000)
