Cell biology Flashcards
Eukaryotes
Eu means true and Karyon means nucleus
DNA is contained wihtin a membrane bound nucleus
- arranged as multiple chromosomes
Organelles are present and membrane-bound
Can be either single celled or multicellular
- most eukaryotes are multicellular
Includes protists (no longer considered a group of eukaryotes, easier to fit them into smaller groups), fungi, plants and animals
What do eukaryotes include?
Fungi, Plants and animals
DNA of Eukaryotes
Dna is contained iwthin a membrane bound nucleus
- arranged as multiple chromosomes
Organelles of eukaryotes
Present and membrane bound
Are eukaryotes multicellular or unicellular
Can be either unicellular or mutlicellular, but most euk. are mutlicellular
Prokaryotes
Pro means before, karyon means nucleus
- DNA is not enclosed within a membrane
- chromosome is single and circular and exists within a specific region of the cell called the nucleoid
They do not have membrane bound organelles such as mitochondria
Single celled organisms only
- bacteria and archaea
Prokaryotes include
Bacteria and archaea
DNA of prokaryotes
DNA is not encolsed within a membrane
- chromosomes are single and circular - exist in a specific region of the called called the nucleoid
What do prokaryotes lack that eukaryotes have?
Prokaryotes do not have membrane bound organelles such as mitochondria and the DNA isn’t membrane bound, so prokaryotes DNA is in a region called the nucleoid
- they are also single celled only! (includes bacteria and archaea
Morphology means…?
- what are the different morphologies
means shape
Shapes: Bacillus, coccus (diplococci, streptococci and staphylococci), and spirillum (spirochete and spirilla)
What are are the different bacterial morphologies and their examples
- Coccus: spherical
- Diplococci - 2 cocci
- Staphylococci - looks like grape
- Streptococcus - coccus arranged in chains
ex. Streptococcus pyogenes - Bacillus: rods
ex.Escherichia coli aka E.coli - Spirillum: Spiral
- Spirochete: long, flexible
ex. Treponema pallidum
Streptococcus pyogenes shape
Streptococcus (a cocci shape)
Coccus and the different cocci, provide an example
Spherical
- Diplococci
- Staphylococcus
- Streptococcus
ex. Streptococcus pyogenes or Streptococcus mutans (capsulated and in our teeth)
Escherichia coli shape
Bacillus (rods)
Bacillus
Rods
ex. Escherichia coli
Treponema pallidum shape
Spirillum
Spirillum
Spiral
- Spirochete: long, flexible
- Hellical shaped organism: typically short, rigid, not a lot of flexibility
ex. Treponema pallidum
Name the outermost layer of bacterium
Glycocalyx (sugar coat)
Glycocalyx
Sugar coat
- gelatinous, sticky polymer (if sticky better chance to stick to surface)
- Can be composed of either polysaccaride, protein or both
- when its made of only sugar, its called an extracellular polysaccaride
- Secreted from the prokaryote onto the outside of the cell wall
Arrangement could be different so there’s two different kinds, can be either a capsule or slime layer
When the glycocalyx is only a sugar then what is it?
An extracellular polysaccaride
What are the two different kinds of glycocalyx
The way they’re going to be arranged is going to be different
1. Capsule: when the substance of the glycocalyx is firmly attached to the cell wall and organized (organized sugar layer - tight on surface)
2. Slime layer: when the substance of the glycocalyx is disorganized and only loosely attached to the cell wall
Capsule
when the substance of the glycocalyx is firmly attached to the cell wall and organized (organized sugar layer - tight on the surface)
Capsules contribute to an organisms virulence
- does so by protecting the organism from phagocytosis (= important for immune defense)
- Certain organisms such as Bacillus anthracis can only cause disease if they are encapsulated
The capsule also allows the organism to adhere to and colonize host cells (eating a lot, replicating a lot, producing a lot of acids when metabolizing)
Also protects the bacterial cell against dehydration and holds nutrients inside the cell
explain virulence and capsules
is the ability to cause disease
- the capsule of an organism contributes to their virulence b/c the bacteria can adhere and colonize host cells
Streptococcus mutans are an example of
encapsulated organisms
- Streptococcus mutans attaches to teeth and may cause cavities
- some organisms such as Streptococcus mutans may use their capsule as an energy source, breaking down the sugars when energy sources are low (use the stuff left on your teeth/mouth as nutrients)
Bacillus anthracis
can only cause disease if they are encapsulated (capsules as an external structure)
Slime layer
when the substance of the glycocalyx is disorganized and only loosely attached to the cell wall
- Describe Flagella of prokaryotes
- name the parts of flagella
- name the different flagellar arrangements
- What are flagellar proteins and their purpose?
- The types of flagellar movement and what it depends on
found on some prokaryotic cells
- long, filamentous, used for motility
Composed of three primary parts
1. filament
2. hook
3. basal body
Flagellar arrangements
1. peritrichous
2. monotrichous
3. lophotrichous
4. amphitrichous
Flagellar proteins are used to differentiate between strains of different bacteria
- Example:* E.coli* 0157:H7 (0157 = the strain thats found on cows and the H7 is the type of flagellar protein)
Flagella can rotate either clockwise or counterclockwise
- run/swim and tumble
- Taxis: chemotaxis and phototaxis
- This flagellar movement depends on energy production
3 primary parts of flagella
1. Filament: composed of the circular flagellin
protein (lots) forming a helix around a hollow core
- Is not covered by a sheath
2. Hook: (= flexibility) made of different protein than the flagella (diff protein, diff location, diff function)
3. Basal body: anchors the flagellum (fasten and attached to a more stabilized structure)
Filament
A primary part of flagella (an external structure)
Filament: composed of the circular flagellin
protein forming a helix around a hollow core
* Is not covered by a sheath
Hook
A primary part of flagella (an external structure)
made of different protein than the flagella (diff protein, diff function, diff location)
Basal body
A primary part of flagella (an external structure)
anchors the flagellum to the plasma
membrane and the cell wall
name the different Flagellar arrangements
- Peritrichous: flagella distributed over the entire cell surface
- Monotrichous: single polar flagella
- Lophotrichous: two or more flagella at one or both ends of the cell
- Amphitrichous: a tuft of flagella at each cell end
Peritrichous
A type of flagellar arrangement
- flagella distributed over the entire cell surface
Monotrichous
A type of flagellar arrangement
- single polar flagella
Lophotrichous
A type of flagellar arrangement
- two or more flagella at one or both ends of the cell
Amphitrichous
A type of flagellar arrangement
a tuft of flagella at each cell end
Flagellar proteins
are used to differentiate in ebtween strains of different bacteria
E.coli 0157:H7
0157 = strain thats found in cows
H7 = type of flagellar protein (= differentiate diff strains of diff bacteria)
Explain bacterial motility
- explain run or swim
- explain tumbles
Explain the different types of taxis
The bacterium is able to change direction and speed on its own
- Run or Swim: movement in one direction for a continuous period of time
- Tumbles: Abrupt or random changes in direction
- results when the flagellum changes its direction of rotation (like a car using a wheel)
Being motile allows the bacterium to move away from dangerous enviornments and toward favorable enviornments which is called taxis
types
1. Chemotaxis
2. phototaxis
Bacteria move toward an attractant and away from a repellant
Run (or swim)
Movement in one direction for a continuous period of time
tumbles
Abrupt or random changes in
direction
- Results when the flagellum changes its
direction of rotation
taxis
Bacterial motility
Being motile allows the bacterium to
move away from dangerous environments
and toward favorable environments
Chemotaxis
Movement toward a chemical stimulus
Phototaxis
Movement toward a light stimulus
Pili and Fimbriae are found in? Compare them to flagella and what do both contain?
many gram negative bacteria (used for attachment and made from protein)
Both are:
- hair like appendages that are shorter, thinner and straighter than flagella and are not used for motility
- made of pilin protein
Define Fimbriae
- where can fimbriae be found on the bacteria
- the importance of fimbriae
- provide an example
Found in many gram - cells, and is made of the same pilin protein and the pili
- bacterial cell can contain a few or hundreds
can be all over cell surface or only at poles
Enables the bacterial cell to adhere to surfaces and other bacterial cells (so that they dont get washed away)
- Allow the bacterium to adhere and colonize without fimbriae colonization cannot occur and thus disease does not occur
- ex. Neisseria gonorrhoeae
Neisseria gonorrhoeae is an example of
A bacterial cell that has fimbriae on its surface
What is Pili and the importance of pili?
(pilus-singular)
Longer than fimbriae (made from same pilin protein, not used to specifically stick to anythings- specialized for exchange, “photocopy” info)
- only one or two per cell
these filaments join two bacterial cells in order to transfer DNA in between them in a process called conjugation (horizontal gene transfer)