Cell Bio

Question 1

3 functions of golgi

Answer 1

1. modifies N-oligosaccharide on asparagine
2. adds O-oligosaccharides on serine + threonine
3. ADDS MANNOSE-6-PHOSPHATE to proteins trafficked to lysomes

Question 2

s/s of I cell disease

Answer 2

coarse facial features
clouded corneas
restricted joint movement
high plasma levels of lysosomal enzymes
often fatal in childhood

Question 3

deficiency in I cell disease

Answer 3

lacking N-acetylglucosaminyl-1-phosphotransferase

• can’t tag mannose residies w/ phosphate
• lysosomal enzymes secreted extracellularly

Question 4

What’s a SRP

Answer 4

Signal Recognition Peptide

• abundant, cytosolic ribonucleoprotein
• traffics ribosomal proteins to RER
• if deficienct: proteins accumulate in cytosol

Question 5

3 types of vesicular trafficking proteins

Answer 5

COP-I
COP-II
Clathrin

Question 6

Vimentin stain

Answer 6

connective tissue

Question 7

GFAP (glial fibrillary acid proteins

Answer 7

neuroGlial

Question 8

DesMin

Answer 8

Muscle = desMin think Muscle

Question 9

cytokeratin

Answer 9

epithelial cells

Question 10

neurofilaments

Answer 10

neurons

Question 11

microfilaments (fxn, examples)

Answer 11

smallest
muscle contraction, cytokinesis
i.e. actin

Question 12

intermediate filaments (fxn, examples)

Answer 12

cell structure

vimentin, cytokeratin, laminin, GFAP, neurofilaments, desmin

Question 13

microtubules (fxn, examples)

Answer 13

bigger than microfilaments!
function in movement and mitosis (cell division)
i.e. cilia, mitotic spindle, flagella, axonal trafficing, centrioles

Question 14

microtubule structure

Answer 14

cylinder, w/ helical heterodimers of alpha and beta tubulin

each dimer has 2 GTP

Question 15

motor proteins associated with microtubules (2)

Answer 15

dyenin: retrograde, + to -
kinesin: anterograde: - to +

Question 16

6 drugs that act on microtubules

Answer 16

Microtubles Get Constructed Very Poorly
Mebendazole 
Griseofulvin
Colchicine
Vincristine + Vinblastine
Paclitaxel

Question 17

defective protein in Menkes disease

Answer 17

ATP7A

impaired copper absorption and transport (required for lysyl oxidase in collagen crosslinking)

Question 18

S/S of Menkes

inheritance

Answer 18

x-linked recessive

brittle, “kinky” hair, hypotonia, growth retardation