CB 6

Question 1

Tau tangles

Answer 1

• tau gets hyperphosphorylated
• Tau and MAP2 normally stabilize and enhance the microtubule array in normal nerve cells
• seen in alzheimer’s disease

Question 2

actin microfilaments

Answer 2

• phallacidin conjugated with a green dye labels the actin microfilaments
• MitoTracker red labels mitochondria
• DAPI stains nuclear DNA blue

Question 3

Cytochalasins

Answer 3

inhibit actin polymerization

Question 4

intermediate filamets

Answer 4

cytokeratins, desmin, GFAP, neurofilament protein, nuclear lamin, vimentin

Question 5

cytokeratins

Answer 5

localize to epithelial cells

Question 6

desmin

Answer 6

localize to muscle [smooth and striated]

Question 7

glial fibrillary acidic protein aka GFAP

Answer 7

localize to astrocytic glial cells, cross bridges are infrequent

Question 8

neurofilament protein

Answer 8

localize to neurons, confer great tensile strength to axons, note extensive cross bridges

Question 9

nuclear lamin

Answer 9

localize to nucleus of all cells

Question 10

vimentin

Answer 10

localize to many mesodermal tissues

Question 11

Fatty liver from alcohol

Answer 11

shows keratin intermediate filaments

Question 12

Epidermolysis bullosa simplex

Answer 12

mutations in the keratin 5 or 14 gene prevent keratin from assembling into strong networks, epidermins of skin becomes fragile and easily damaged, blistering of skin

Question 13

Rosenthal’s fibers

Answer 13

aggregates of GFAP and other proteins, characteristic of pilocytic astrocytoma

Question 14

PML- promyelocytic leukemia bodies

Answer 14

assembles and modifies proteins involved in DNA repair and induction of apoptosis

Question 15

Osteogenesis Type 1

Answer 15

results when nuclear RNA is not exported

Question 16

progeria

Answer 16

disease that rapidly accelerates aging

Question 17

Papillary thryoid cancer

Answer 17

will see orphan annie eye nuclei