Catalase Pos. GPR- Corynebacteria & Listeria

Question 1

Corynebacterium diphtheria are irregularly blank shaped because they undergo blank after cell division

Answer 1

V-shaped, snapping movements

Question 2

Generally the cause of death from diphtheria is…

Answer 2

Toxic myocarditis

Question 3

Dtap is the vaccine for…

Answer 3

Protecting against corynebacterium diphtheria

Question 4

The diphtheria pseudomembrane is composed of blank and blank

Answer 4

Dead cells and fibrin matrix

Question 5

Dtap vaccine contains blank which is blank

Answer 5

Diphtheria toxoid, an inactivated toxin

Question 6

Corynebacterium diphtheria contains three distinct pilus structures known as blank blank and blank

Answer 6

SpaA, spaB, spaC

Question 7

Corynebacterium diphtheria adherence factors bind to blank blank cells

Answer 7

Pharyngeal epithelial cells

Question 8

Corynebacterium’s major Pilus protein is…

Answer 8

SpaA

Question 9

Corynebacterium’s minor Pilin proteins are blank and blank

Answer 9

SpaB and spaC

Question 10

Diphtheria toxin is a blank that ADP-ribosyltes blank

Answer 10

A-B exotoxin, elongation factor 2 (EF-two)

Question 11

Diphtheria toxin inhibits blank by ADP-ribosylation of blank

Answer 11

Inhibits protein translation, elongation factor 2

Question 12

Diphtheria toxin is encoded by the blank gene on lysogenic blank

Answer 12

Tox gene, lysogenic beta-phage

Question 13

The three steps involved in diphtheria infections include…

Answer 13

Toxin processing, entry into host cells, activation in the host cytosol

Question 14

The a subunit of diphtheria toxin is considered the blank

Answer 14

Catalytic domain

Question 15

The B subunit of diphtheria toxin is considered the blank

Answer 15

Transmembrane and receptor binding domain

Question 16

That the B subunit binds to blank for entry into the host cell

Answer 16

Heparin binding epidermal growth factor (hb-EGF)

Question 17

Diphtheria toxin are internalized via endocytosis in blank and converted into blank

Answer 17

Catherine coated pits, early endosomal vesicles (EEV)

Question 18

EF2 contains his residue called blank

Answer 18

Dipthamide

Question 19

How does subunit A escape the EEV encapsulating diphtheria toxin

Answer 19

Acidification of the EEV results in refolding of the B subunit transmembrane domain, forms of pore in the membrane, A subunit dissociates and translocates through pore

Question 20

High levels of iron in the host blanks dtxR and toxin production is blanked

Answer 20

Activates, repressed

Question 21

Low levels of iron in the host blanks dtxR and toxin production is blanked

Answer 21

Deactivates, induced

Question 22

Blank is a selective medium for corynebacterium diphtheria. On this medium diphtheria colonies will appear blank

Answer 22

Tisdale medium with tellurite, black brown halo

Question 23

The blank test provide information regarding diphtheria toxin production

Answer 23

ELEK TEST

Question 24

Corynebacterium blank is an opportunistic nosocomial pathogen associated with intravascular devices

Answer 24

Corynebacterium Jeikeium

Question 25

Corynebacterium Jeikeium would not likely be present in blank because it is a blank gram-positive rod

Answer 25

Water supply, lipophilic

Question 26

Listeria can enter the body through blank or blank

Answer 26

Good or water

Question 27

Listeria human pathogen

Answer 27

Listeria monocytogenes

Question 28

Listeria animal pathogen

Answer 28

Listeria Ivanovii

Question 29

Wisteria can cause blank blank and blank in newborns elderly and immunocompromise individuals

Answer 29

Listeriosis, sepsis, meningitis

Question 30

Two portals of entry into intestinal lumen include blank and blank

Answer 30

E-Cadherin and goblet cells

Question 31

Blank is a major problem in food safety

Answer 31

Cold enrichment, ability to grow at low temperatures

Question 32

Describe Listeria Monocytongenes oxygen requirements

Answer 32

Facultative intracellular pathogen

Question 33

Wisteria uses the blank mechanism for invasion into host

Answer 33

Zipper mechanism

Question 34

Listeria binds to blank and blank proteins on the host cell surface

Answer 34

InlA and InlB

Question 35

Listeria lyse the phagocytic vacuole by using pore forming toxin blank and membrane disrupting blank and blank

Answer 35

LLO, PI-PLC, PC-PLC

Question 36

LLO is a...

Answer 36

Cholesterol dependent cytolysin

Question 37

LLO can enter host cells through binding to...

Answer 37

TLR-4

Question 38

What 3 components allow listeria to evade immune system during dissemination

Answer 38

Cell to cell spreading Surface protein ActA Actin polymerization

Question 39

Many of listeria virulence factors are encoded in blank and controlled by master regulator blank

Answer 39

Listeria pathogenicity island-1 (LPI-1), PrfA

Question 40

PrfA is a transcriptional blank

Answer 40

Activator

Question 41

Transcription of PrfA is induced by blank and repressed by blank

Answer 41

Stress signals, sugars

Question 42

Translation of PrfA is regulated by blank

Answer 42

Riboswitches, I.e. Thermoregulation

Question 43

Describe translation of PrfA at room temp

Answer 43

The confirmation of mRNA prevents ribosome binding to RBS

Question 44

Describe translation of PrfA at physiological temperature

Answer 44

mRNA opens up to allow ribosome binding to RBS and translation occurs

Question 45

How does listeria import iron

Answer 45

In the form of Fe 3+ because it does not make siderophores

Question 46

Listeria motility mechanism at room temp

Answer 46

Peritricous flagella

Question 47

Listeria motility mechanism at physiological temp

Answer 47

Actin recruitment

Question 48

Corynebacterium are also referred to as blank

Answer 48

Bacterium diphtheroids