Catalase Pos. GPR- Corynebacteria & Listeria Flashcards
Corynebacterium diphtheria are irregularly blank shaped because they undergo blank after cell division
V-shaped, snapping movements
Generally the cause of death from diphtheria is…
Toxic myocarditis
Dtap is the vaccine for…
Protecting against corynebacterium diphtheria
The diphtheria pseudomembrane is composed of blank and blank
Dead cells and fibrin matrix
Dtap vaccine contains blank which is blank
Diphtheria toxoid, an inactivated toxin
Corynebacterium diphtheria contains three distinct pilus structures known as blank blank and blank
SpaA, spaB, spaC
Corynebacterium diphtheria adherence factors bind to blank blank cells
Pharyngeal epithelial cells
Corynebacterium’s major Pilus protein is…
SpaA
Corynebacterium’s minor Pilin proteins are blank and blank
SpaB and spaC
Diphtheria toxin is a blank that ADP-ribosyltes blank
A-B exotoxin, elongation factor 2 (EF-two)
Diphtheria toxin inhibits blank by ADP-ribosylation of blank
Inhibits protein translation, elongation factor 2
Diphtheria toxin is encoded by the blank gene on lysogenic blank
Tox gene, lysogenic beta-phage
The three steps involved in diphtheria infections include…
Toxin processing, entry into host cells, activation in the host cytosol
The a subunit of diphtheria toxin is considered the blank
Catalytic domain
The B subunit of diphtheria toxin is considered the blank
Transmembrane and receptor binding domain
That the B subunit binds to blank for entry into the host cell
Heparin binding epidermal growth factor (hb-EGF)
Diphtheria toxin are internalized via endocytosis in blank and converted into blank
Catherine coated pits, early endosomal vesicles (EEV)
EF2 contains his residue called blank
Dipthamide
How does subunit A escape the EEV encapsulating diphtheria toxin
Acidification of the EEV results in refolding of the B subunit transmembrane domain, forms of pore in the membrane, A subunit dissociates and translocates through pore
High levels of iron in the host blanks dtxR and toxin production is blanked
Activates, repressed
Low levels of iron in the host blanks dtxR and toxin production is blanked
Deactivates, induced
Blank is a selective medium for corynebacterium diphtheria. On this medium diphtheria colonies will appear blank
Tisdale medium with tellurite, black brown halo
The blank test provide information regarding diphtheria toxin production
ELEK TEST
Corynebacterium blank is an opportunistic nosocomial pathogen associated with intravascular devices
Corynebacterium Jeikeium
Corynebacterium Jeikeium would not likely be present in blank because it is a blank gram-positive rod
Water supply, lipophilic
Listeria can enter the body through blank or blank
Good or water
Listeria human pathogen
Listeria monocytogenes
Listeria animal pathogen
Listeria Ivanovii
Wisteria can cause blank blank and blank in newborns elderly and immunocompromise individuals
Listeriosis, sepsis, meningitis
Two portals of entry into intestinal lumen include blank and blank
E-Cadherin and goblet cells
Blank is a major problem in food safety
Cold enrichment, ability to grow at low temperatures
Describe Listeria Monocytongenes oxygen requirements
Facultative intracellular pathogen
Wisteria uses the blank mechanism for invasion into host
Zipper mechanism
Listeria binds to blank and blank proteins on the host cell surface
InlA and InlB
Listeria lyse the phagocytic vacuole by using pore forming toxin blank and membrane disrupting blank and blank
LLO, PI-PLC, PC-PLC
LLO is a…
Cholesterol dependent cytolysin
LLO can enter host cells through binding to…
TLR-4
What 3 components allow listeria to evade immune system during dissemination
Cell to cell spreading
Surface protein ActA
Actin polymerization
Many of listeria virulence factors are encoded in blank and controlled by master regulator blank
Listeria pathogenicity island-1 (LPI-1), PrfA
PrfA is a transcriptional blank
Activator
Transcription of PrfA is induced by blank and repressed by blank
Stress signals, sugars
Translation of PrfA is regulated by blank
Riboswitches, I.e. Thermoregulation
Describe translation of PrfA at room temp
The confirmation of mRNA prevents ribosome binding to RBS
Describe translation of PrfA at physiological temperature
mRNA opens up to allow ribosome binding to RBS and translation occurs
How does listeria import iron
In the form of Fe 3+ because it does not make siderophores
Listeria motility mechanism at room temp
Peritricous flagella
Listeria motility mechanism at physiological temp
Actin recruitment
Corynebacterium are also referred to as blank
Bacterium diphtheroids
