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Clinical Micro > Catalase Pos. GPR- Corynebacteria & Listeria > Flashcards

Catalase Pos. GPR- Corynebacteria & Listeria Flashcards

1
Q

Corynebacterium diphtheria are irregularly blank shaped because they undergo blank after cell division

A

V-shaped, snapping movements

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2
Q

Generally the cause of death from diphtheria is…

A

Toxic myocarditis

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3
Q

Dtap is the vaccine for…

A

Protecting against corynebacterium diphtheria

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4
Q

The diphtheria pseudomembrane is composed of blank and blank

A

Dead cells and fibrin matrix

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5
Q

Dtap vaccine contains blank which is blank

A

Diphtheria toxoid, an inactivated toxin

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6
Q

Corynebacterium diphtheria contains three distinct pilus structures known as blank blank and blank

A

SpaA, spaB, spaC

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7
Q

Corynebacterium diphtheria adherence factors bind to blank blank cells

A

Pharyngeal epithelial cells

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8
Q

Corynebacterium’s major Pilus protein is…

A

SpaA

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9
Q

Corynebacterium’s minor Pilin proteins are blank and blank

A

SpaB and spaC

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10
Q

Diphtheria toxin is a blank that ADP-ribosyltes blank

A

A-B exotoxin, elongation factor 2 (EF-two)

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11
Q

Diphtheria toxin inhibits blank by ADP-ribosylation of blank

A

Inhibits protein translation, elongation factor 2

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12
Q

Diphtheria toxin is encoded by the blank gene on lysogenic blank

A

Tox gene, lysogenic beta-phage

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13
Q

The three steps involved in diphtheria infections include…

A

Toxin processing, entry into host cells, activation in the host cytosol

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14
Q

The a subunit of diphtheria toxin is considered the blank

A

Catalytic domain

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15
Q

The B subunit of diphtheria toxin is considered the blank

A

Transmembrane and receptor binding domain

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16
Q

That the B subunit binds to blank for entry into the host cell

A

Heparin binding epidermal growth factor (hb-EGF)

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17
Q

Diphtheria toxin are internalized via endocytosis in blank and converted into blank

A

Catherine coated pits, early endosomal vesicles (EEV)

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18
Q

EF2 contains his residue called blank

A

Dipthamide

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19
Q

How does subunit A escape the EEV encapsulating diphtheria toxin

A

Acidification of the EEV results in refolding of the B subunit transmembrane domain, forms of pore in the membrane, A subunit dissociates and translocates through pore

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20
Q

High levels of iron in the host blanks dtxR and toxin production is blanked

A

Activates, repressed

21
Q

Low levels of iron in the host blanks dtxR and toxin production is blanked

A

Deactivates, induced

22
Q

Blank is a selective medium for corynebacterium diphtheria. On this medium diphtheria colonies will appear blank

A

Tisdale medium with tellurite, black brown halo

23
Q

The blank test provide information regarding diphtheria toxin production

A

ELEK TEST

24
Q

Corynebacterium blank is an opportunistic nosocomial pathogen associated with intravascular devices

A

Corynebacterium Jeikeium

25
Q

Corynebacterium Jeikeium would not likely be present in blank because it is a blank gram-positive rod

A

Water supply, lipophilic

26
Q

Listeria can enter the body through blank or blank

A

Good or water

27
Q

Listeria human pathogen

A

Listeria monocytogenes

28
Q

Listeria animal pathogen

A

Listeria Ivanovii

29
Q

Wisteria can cause blank blank and blank in newborns elderly and immunocompromise individuals

A

Listeriosis, sepsis, meningitis

30
Q

Two portals of entry into intestinal lumen include blank and blank

A

E-Cadherin and goblet cells

31
Q

Blank is a major problem in food safety

A

Cold enrichment, ability to grow at low temperatures

32
Q

Describe Listeria Monocytongenes oxygen requirements

A

Facultative intracellular pathogen

33
Q

Wisteria uses the blank mechanism for invasion into host

A

Zipper mechanism

34
Q

Listeria binds to blank and blank proteins on the host cell surface

A

InlA and InlB

35
Q

Listeria lyse the phagocytic vacuole by using pore forming toxin blank and membrane disrupting blank and blank

A

LLO, PI-PLC, PC-PLC

36
Q

LLO is a…

A

Cholesterol dependent cytolysin

37
Q

LLO can enter host cells through binding to…

A

TLR-4

38
Q

What 3 components allow listeria to evade immune system during dissemination

A

Cell to cell spreading
Surface protein ActA
Actin polymerization

39
Q

Many of listeria virulence factors are encoded in blank and controlled by master regulator blank

A

Listeria pathogenicity island-1 (LPI-1), PrfA

40
Q

PrfA is a transcriptional blank

A

Activator

41
Q

Transcription of PrfA is induced by blank and repressed by blank

A

Stress signals, sugars

42
Q

Translation of PrfA is regulated by blank

A

Riboswitches, I.e. Thermoregulation

43
Q

Describe translation of PrfA at room temp

A

The confirmation of mRNA prevents ribosome binding to RBS

44
Q

Describe translation of PrfA at physiological temperature

A

mRNA opens up to allow ribosome binding to RBS and translation occurs

45
Q

How does listeria import iron

A

In the form of Fe 3+ because it does not make siderophores

46
Q

Listeria motility mechanism at room temp

A

Peritricous flagella

47
Q

Listeria motility mechanism at physiological temp

A

Actin recruitment

48
Q

Corynebacterium are also referred to as blank

A

Bacterium diphtheroids

Clinical Micro (4 decks)

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