Case Studies Flashcards
There is no question as to maternity. Why is the mother’s DNA analyzed?
a. The mother’s alleles must be compared to the father’s allels.
b. The mother’s alleles must be proven to be different from the father’s alleles.
c. The mother’s alleles have to be identical to all of the child’s alleles.
d. The heir’s alleles not shared with the mother will have to be provided from the father.
d. The heir’s alleles not shared with the mother will have to be provided from the father.
In diploid organisms, one of each chromosome pair (carrying one allele) is inherited for each parent. Half of the alleles in the heir will be the same as half of the mother’s alleles. The other alleles will come from the father.
How are alleles determined by PCR and capillary electrophoresis?
a. The size (migration speed) of the PCR products
b. The areas under the fluorescent peaks on the electropherogram.
c. The height of the fluorescent peaks on the electropherogram.
d. Restriction enzyme sites in each PCR product.
a. The size (migration speed) of the PCR products
For STR analysis by PCR, the primers are designed to produce a product containing the STR sequences. The size (migration speed in the capillary) of each PCR product will be dependent on the number of repeats it contains. The areas and heights of the peaks are not associated with the identity of the alleles.
One of the loci tested in the analysis was the amelogenin locus. There was one strong peak at this locus in the heir’s DNA. What does this indicate about the heir?
a. More than one peak is necessary to interpret any locus.
b. The putative heir is female.
c. The putative heir is male.
d. Paternity by the financier is unlikely.
b. The putative heir is female.
The amelogenin loocus is not an STR but a gene located on the X and Y chromosomes. The allele on the Y chromosome is slightly larger than that on the X chromosome. Since females are homozygous for X, only one peak will be present.
It is very important in legal cases such as this to unambiguously identify alleles. What cotnrols are used to ensure the accurate comparison of migration speeds in each capillary?
a. Positive control from a cell line.
b. amplification control
c. amelogenin loci, which are always the same size
d. internal lane standards
d. internal lane standards
Internal lane standards migrate through the capillary with the PCR prodcuts containing the STR. The capillary instrument assess their size (migration speed) by direct comparison with the standards of known size.
Twelve loci were tested. The results of the PCR analysis revealed that half of the alleles of the putative heir were the same as half of the alleles of the financier. What does this mean?
a. The financier is excluded as the father.
b. The financier is 50% included as the father.
c. Depending on the allele frequencies, there is a very high probability of paternity.
d. All alleles must match in order to prove paternity with high confidence.
c. Depending on the allele frequencies, there is a very high probability of paternity.
A paternity test is designed to choose between two hypotheses: The test subject is not the father of the tested child (Ho), or the test subject is the father of the tested child (H1). Paternity is first assessed by observation of shared alleles between the alleged father and child. Note that half of each parent’s alleles are expected to match in the case of positive paternity. If at least one paternal allele allele does not match, the alleled father is excluded. Results are not reported as 50% certain, nor as completely certain, leaving the possibility that someone else may be the parent.
Why is the PCR method preferred for this application?
a. PCR is more labor intensive than other methods.
b. The PCR method has fewer artifacts than Southern blot.
c. PCR can be performed on degraded specimens.
d. STR allelels can be analzed only by PCR
b. The PCR method has fewer artifacts than Southern blot.
Since PCR can be performed on a template of a few hundred base pairs, it is the preferred method for specimens that are likely to have fractured DNA. The chance of a chromosome break within the small area of the PCR target is much less than in the hundreds to thousands of base pair RFLP fragments analyzed by Southern blot. STR can be assessed by methods other than PCR—for example, RFLP or direct sequencing. These methods are not practical for limited specimens, however.
Why were the donor and recipient tested prior to the transplant?
a. This test is used to select a suitable donor.
b. This pretransplate testing is performed to identify the donor the recipient.
c. STR must match in order for the transplant to be successful.
d. Pretransplant testing is required to identify STR alleles that differ between the donor and recipient.
d. Pretransplant testing is required to identify STR alleles that differ between the donor and recipient.
Engraftment analysis after a transplant depends on the ability to distinguish the donor from the recipient. In order to do this, STR loci must be analyzed in the donor and the recipient before the recipient receives donor cells. If this is not done, alleles observed in the recipient after transplant cannot be assigned to donor or recipient. The pretransplant analysis is not used to select a donor. The donor is selected using tissue typing or HLA analysis. The STR does not affect the engraftment. The test is not used for human identification, as the alleles are surveyed for informativity (different between donor and recipient) without necessarily identifying specific alleles.
In what case might the pretransplant STR analysis yield no informative loci?
a. The donor and recipient are fraternal twins.
b. The donor and recipient are identical twins.
c. The donor is homozygous for all STR alleles tested.
d. The recipient is a part of the donor.
b. The donor and recipient are identical twins.
Pretransplant STR analysis will not produce informative loci in the case of identical twins. Unlike dizygotic (fraternal) twins, monozygotic twins from a single fertilized egg share the same DNA and therefore the same STR alleles. Even closely related individuals (siblings or parents) will have different alleles among the STR loci tested. Informativity of loci is not dependent on whether the loci are homozygous or heterozygous. In the case of identical twins, STR analysis cannot be used for post-transplant engraftment analysis.
What cell fraction expressed CD3?
a. granulocytes
b. myeloid cells
c. B cells
d. T cells
d. T cells
CD3 is a membrane receptor protein expressed on T cells. It is used to select the T-cell population from the rest of the white blood cells. T cells are factors in graft-versus-host disease, which can affect the health of the recipient after the transplant. The T-cell fraction also participates in a graft-versus-tumor effect that is beneficial for preventing relapse of malignancies. Other fractions such as myeloid cells may also be tested separately for engraftment. B cells are not usually tested as a separate cell fraction.
What term is used to indicate different degrees of engraftment among different cell fractions - for example, T-cells versus myeloid cells?
a. full chimerism
b. incomplete chimerism
c. spilt chimerism
d. graft failure
c. spilt chimerism
Not all cell fractions engraft with equal kinetics. For example, granulocytes may show all donor alleles, while T cells in the same patient may be a mixture of donor and recipient alleles.
Full chimerism indicates complete engraftment of all fractions.
Graft failure indicates decreasing levels of loss of donor alleles.
Incomplete Chimerism is not a term that is used.
The results of the transplant at the D16S539 locus were similar to those shown below. There are both donor and recipient STR alleles in the recipient following transplant. How is the degree of engraftment (percent donor) quanitified?
a. compare peak heights of the D16S539 PCR product in the recipient pre- and post-transplant.
b. Divide the area under the donor peak(s) with total peak area at the D16S539 locus, ignoring shared alleles
c. divide the area under the donor peak(s) by the area under the recipient peak(s) at the D16S539 locus.
d. divide the area under the donor peak(s) by the area under the pretransplant donor peak(s) at the D16S539) locus.
b. Divide the area under the donor peak(s) with total peak area at the D16S539 locus, ignoring shared alleles.
To quantify percent donor, add the area under the donor peak(s) in fluorescence units (provided by the instrument software). Then determine the total peak area by adding the area under the donor + recipient peak(s). Divide the donor peak area by the total peak area (not by the recipient peak area) and multiply by 100 to get the percentage. In the example, one of the peaks is the same in the donor and recipient. Its area is not included in the calculation. Instead of area, the heights of the peaks may be used in a similar manner. Pretransplant peaks are run for reference only to confirm the location of the donor and recipient alleles.
What is the interpretation is two peaks from different sources align with the same migration speed or distance (bin) in the capillary analysis?
a. the peaks represent the same allele
b. an error has occurred, as peaks from different sources should migrate at different speeds
c. in order for peaks to be interpreted as the same allele, the peak heights must be the same.
d. the peaks represent degraded PCR products
a. the peaks represent the same allele
STR analysis is performed by amplifying a short region of DNA containing the STR. The size of the PCR products reflects the number of tandem repeats in the STR. PCR products of equal size have the same number of tandem repeats and are interpreted as the same allele. PCR products of the same STR locus from two different sources may or may not be of the same size. Degraded PCR products will not consistently resolve at any particular migration speed.
The alleles detected in the TH01 STR locus were 7,10. What do the numbers 7 and 10 denote?
a. The relative allele frequency of the two alleles
b. the number of nucleotides in each allele
c. the number of tandem repeats in each allele
d. the chromosome on which the alleles are found
c. the number of tandem repeats in each allele
Short tandem repeat alleles are distinguished by the number of repeats in each allele. For THO1, located on chromosome 11, the repeated sequence is TCAT. There are seven repeats of the 4-bp sequence, TCAT on one of the two chromosomes 11. There are 10 TCAT repeats on the other chromosome 11. Only in the case of single-bp repeats would the numbers indicate the number of nucleotides in each STR. The actual number of base pairs in an allele analyzed by PCR (or RFLP) is determined by the size of the fragment generated from the primers or restriction enzymes used. Allele frequency is the fraction of people in the population sharing that allele and is expressed as a number between 0 and 1.
There are two alleles at most of the loci in this genotype. There is one allele at the D12S579 locus. What does this indicate?
a. a PCR failure occurred on only one allele amplified
b. the individual has only one chromosome 12
c. the genotype is not complete and must be reanalyzed
d. the individual is homozygous at the D12S579 locus
d. the individual is homozygous at the D12S579 locus
If an individual inherits different alleles from each parent, the locus will be heterozygous. If an individual inherits the same allele from each parent, the individual will be homozygous for that locus. Laboratory error (PCR failure, incomplete genotype) can be ruled out, since all other loci and controls are successfully amplified and since the chance of PCR failure at only those loci matching a homozygous loci in a genotype from an independent source (the database) is small.
The alleles matched at 13 loci. What does this mean?
a. The alleles detected are very frequent in the human population
b. the individual in the database cannot be excluded as a suspect
c. the individual in the database cannot be included as a suspect
d. the alleles detected are rare in the human population
b. the individual in the database cannot be excluded as a suspect
The matching loci are sufficient for legal identification. The genotype found at the crime scene belongs to the subject in the database, or his identical twin. A matching genotype such as in this case is dependent on, but does not indicate, the frequency or rarity of the matching alleles. Frequently occurring alleles may be found more often in unrelated individuals; however, the probability of two unrelated individuals having the same set of 13 alleles is less than 1/1,000,000,000,000.
What is the purpose of the label on the primers?
a. Only specific HLA types will be amplified and therefore labeled.
b. All amplicons must be labeled for later detection.
c. The label is required for binding with the probes.
d. The labe prevents mispriming in the PCR reaction.
b. All amplicons must be labeled for later detection.
Hybridization of a labeled probe to immobilized amplicons of the HLA genes (dot blot) was one of the first methods that utilized polymerase chain reaction (PCR)-amplified DNA for HLA typing. Conversely, unlabeled probes can be immobilized and hybridized to labeled test specimen. The test amplicons are labeled using tailed primers with a colorimetric, chemiluminescent or fluorescent tag. This addition to the 5’ end of a primer will not determine its target specificity nor prevent mispriming. The amplicons from each donor sample will hybridize only to the probes complementary to their specific allele. The donor allele is then determined according to which position on the membrane array shows fluorescence. The fluorescent molecules are required for identification of which probe is binding the sample amplicon. They are not required for hybridization.
How are allele-specific probes distinguished from one another?
a. length in base pairs
b. the number of probes used
c. the position of hybridization on the membrane
d. fluorescent wavelength
c. the position of hybridization on the membrane
The donor allele is determined according to which position on the membrane array shows fluorescence. All test amplicons will have the same fluorescent wavelength. Probe lengths (19 to 20 bases) are not associated with any specific allele. The number of probes used depends on the design of the assay. For example, an intermediate resolution assay of the HLA-DRB locus might take 30 to 60 probes. Studies have achieved high-resolution identification of the majority of HLA-A, B, and C alleles using 67 HLA-A, 99 HLA-B, and 57 HLA-C probes and intermediate resolution with 39 HLA-A and 59 HLA-B alleles.
Where is the optimal position for the polymorphic nucleotide in the probe sequence?
a. either end
b. both ends
c. 5’ end only
d. middle
d. middle
The probe sequences are aligned so that the polymorphic nucleotide is in the middle of the probe sequence. Hybridization conditions depend on the optimal binding of the probe matching a test sequence in comparison with another sequence, differing from (not complementary to) the probe by at least one base. Polymorphisms on the ends of the probe molecule may not consistently destabilize the hybridization of a nonmatching amplicon.
In the SSOP assay, a blue colorimetric signal is seen at probe position E3 (A*03:02). Blue color was not observed at any other position (except positive controls), including probe at position D5 (A*26.10). How is this interpreted?
a. the probe at position D5 is defective
b. the probe at position E3 is contaminated
c. the HLA-A type is A*3:02
d. The HLA-A type is A*26:10
c. the HLA-A type is A*3:02
Each allele-specific oligomer probe will hybridize only to its labeled matching amplicon. Probe sequences are based on sequence alignments of HLA polymorphic regions. Association of the amplicon signal with a probe position on the membrane or plate well indicates presence of that allele in the test sample.
Which of the following donors is capatible with the recipient?
a. Donor A
b. Donor B
c. Donor C
d. Donor D
a. Donor A
Donor A, A*03:03/A*09:02, B*39:06, DRB1*07:02/DRB1*08:17 is the best match to the patient type, A*09:02, B*39:06, DRB1*07:02/DRB1*08:17. The donor is heterozygous at the HLA-A locus but carries the A*09:02 allele of the recipient. Both donor and recipient are homozygous for the B*39:06 allele at HLA-B and carry the DRB1*07:02/DRB1*08:17 genotype at HLA-DR. None of the other donors share as many alleles with the recipient. SSOP is considered low to intermediate resolution, depending on the number and types of probes used in the assay. As some probes have multiple specificities, hybridization panels can be complex. Computer programs may be used for accurate interpretation of SSOP results.
What is the reason to test for antihuman antibodies?
a. antihuman antibodies may react against a donor organ
b. antihuman antibodies facilitate finding a compatible donor
c. all serum contains antihuman antibodies
d. antihuman antibodies sensitize patients to therapy
a. antihuman antibodies may react against a donor organ
Correct Answer: 1.
Successful organ transplant depends on minimal reaction of the recipient’s immune system to the antigens of the donor organ. Normally, serum does not contain antibodies against human antigens. However, persons who have had pregnancies, a previous organ transplant, or blood transfusions will have antihuman antibodies (termed humoral sensitization) that may react against a new donor organ. These antibodies do not facilitate finding organ donors, nor do they have any effect on therapy.
What is one reason the presence of antihuman antibodies is predicted in this case?
a. the patient has not had a previous organ transplant
b. the patient has several children
c. the patient is female
d. the patient is over 40 years of age
b. the patient has several children
Persons who have had a previous organ transplant, blood transfusions, or pregnancies have antihuman antibodies (termed humoral sensitization) that may react against a new donor organ. Antihuman antibodies are not induced by gender or age.
The flow cytometry test for PRA was performed with microbeads. What is the advantage of using microbeads?
a. microbeads are less expensive than reagents required for the CDC test.
b. this method identifies specific antigens using pooled antibodies
c. the instrument for this test is available in all laboratories
d. there is less subjectivitiy in interpretation of results
d. there is less subjectivitiy in interpretation of results
Screening of sera with microparticles (beads) is performed in laboratories with flow cytometry capability. For this method, microparticles are attached to pools of antigens derived from cell lines. The microparticles are exposed to test serum, and beads carrying antigens that are recognized by antibodies present in the test serum will bind to those antibodies. After removal of unbound antibodies, a fluorescently labeled secondary reporter antibody is applied, and the antibody-bound beads are detected by flow cytometry. The advantages of this method over the CDC test are that the reaction is performed in a single tube, and there is less subjectivity in the interpretation of results. Because this test uses pooled antigens, however, it can detect prevalence of antihuman antibodies in the test serum but not identify which specific antibodies are present. A negative result does preclude further antihuman antibody assessment.
In the flow cytometry method, what is covalently attached to the microbeads?
a. human antigens
b. human antibodies
c. other microparticles
d. fluorescent labels
a. human antigens
Screening of sera with microparticles (beads) is performed using microparticles attached to pools of antigens derived from cell lines of defined HLA types. When the microparticles are exposed to test serum, beads carrying antigens will bind to antibodies, if present, in the test serum. A fluorescently labeled secondary reporter antibody is then applied, and the antibody-bound beads are detected by flow cytometry. Particles without bound antigen (bound to other particles or antibodies) would not bind antibodies in the test serum. The fluorescent label will only be associated with beads that have bound serum antibody.
What do the results of the test (20% PRA) indicate?
a. it will be impossible to find a donor for this patient
b. no antihuman antibodies are present in the patient serum
c. only 20% of potential donor organs will be compatible
d. potential donors may be found for this patient
d. potential donors may be found for this patient
The percentage of the panel reactive antibodies in the test specimen would bind to the tissue types of 20% of the people in the population. Crossmatch of negative donors may therefore be found for this patient. Patients with %PRA activity of more than 50% are considered to be highly sensitized; finding crossmatch-negative donors is more difficult in these cases.
What control tests the lower limit of detection in each assay run?
a. amplification
b. positive
c. sensitvity
d. negative
c. sensitivity
The sensitivity control should be at or near the lowest point of the AMR. If the sensitivity control does not consistently produce the expected signal, the AMR and the test conditions used must be reviewed and reestablished if necessary. The positive control should always produce positive signal at the high end of the AMR, while the amplification control should give a robust signal from an independent target. There should be no signal from the negative control.
Which sample measurements will not be acceptable if the lower limit of detection is not being reached?
a. high positive sample results
b. negative sample results
c. high positive control
d. amplification control
b. negative sample results
Virus present at the lower limit of detection may be missed if the procedure is not producing signal from the analyte at that level. This will result in false-negative results. When the low positive control doesn’t produce signal, then all negative sample results are in question. In a quantitative assay, the levels of virus in positive samples may also be questioned, especially if the high positive control values are less than expected. Since the amplification control in this example is an independent target, its detection should not be affected.
What part of the test development process in this example is not designed properly?
a. AMR measurement
b. validation
c. calibration
d. amplification controls
a. AMR measurement
The AMR is established from undiluted specimens or a known concentration of analyte in the appropriate matrix. In this example, the synthetic viral genomes were prepared in water, rather than normal plasma, which would be the proper matrix. Validation is performed by comparing results from the new assay to blinded results from a previously validated procedure or an established reference standard. Calibration is performed to determine the input-response capability of the instrument using defined calibrators. A variety of amplification controls may be used for qPCR assays, including human genes.