Calculations Flashcards
What type of verification method is used in the maintenance/calibration of pipets?
gravimetric method (verifies accuracy of the amount of water dispensed by the pipet - weight is equal to volume dispensed)
Name two methods to calibrate pipets?
gravimetric (weight of water is proportional to the volume dispensed) AND volumetric (dye pipetted into specific volume that is then verified by spectrophotometry)
What is considered room temperature?
20 - 24 Celsius
What is the temperature for a general laboratory refrigerator?
2 - 8 Celsius
What is the temperature for a blood bank refrigerator?
1 - 6 Celsius
What is the temperature for a general laboratory freezer?
greater than or equal to -18 Celsius
What is the temperature for a blood bank freezer?
greater than or equal to -65 Celsius
What is the general temperature of a laboratory heat block?
36 - 38 Celsius
What is included as part of maintenance for microscopes?
daily cleaning of the microscope
Name one use for phase contrast microscopy.
manual platelet counts
Name one use for dark field microscopy.
identification of spirochetes
Name one use for polarized microscopy.
identification of urine crystals
What is the size of the eyepiece or ocular of a microscope?
10x objective
What is the function of the condenser of a microscope?
the movable part used to focus light onto the specimen
What is the size of the high powered (non-oil) objective?
40x
What is the function of the stage on a microscope?
hold the slide in place
When do you use the fine adjustment knob on a microscope?
focus under high power
When would one use the course adjustment knob on a microscope?
focus under low power
How should one carry a microscope?
by the base and arm
What is the purpose of the iris diaphragm on a microscope?
used to regulate the light intensity and size of the cone of light that is projected upward toward the slide
What is the size of oil immersion objectives?
between 50x and 100x
Describe the five SI Units of measure.
- Meter 2. kilogram 3. second 4. kelvin 5. mole
What is the scientific notation for the SI prefix “milli”?
10^-3 (milli -3)
What is the scientific notation for the SI prefix “femto”?
10^-15 (femto -15)
What is the scientific notation for the SI prefix “micro”?
10^-6 (micro -6)
What is the scientific notation for the SI prefix “Deci”?
10^-1 (deci -1)
What is the scientific notation for the SI prefix “Centi”?
10^-2 (centi -2)
What is the scientific notation for the SI prefix “Nano”?
10^-9 (nano -9)
What is the scientific notation for the SI prefix “Pico”?
10^-12 (Pico -12)
What is one way to remember the SI Prefixes?
Deci -1, Centi -2, Milli -3, Micro -6, Nano -9, Pico -12, femto -15
What is the scientific notation for the SI prefix “Deka”?
10^1 (deka 1)
What is the scientific notation for the SI prefix “Hecto”?
10^2 (hector 2)
What is the scientific notation for the SI prefix “kilo”?
10^3 (kilo 3)
What is the scientific notation for the SI prefix “mega”?
10^6 (mega 6)
What is the scientific notation for the SI prefix “giga”?
10^9 (giga 9)
What is the temperature conversion for Celsius?
Celsius = 5/9 (Fahrenheit - 32)
What is the temperature conversion for Fahrenheit?
Fahrenheit = (9/5) Celsius = 32
What is the temperature conversion for Kelvin?
Kelvin = Celsius + 273