Carcinogenesis - Molecular Hallmarks of Cancer Cells Flashcards
What is carcinogenesis?
The formation of cancer.
What 2 steps are necessary for neoplasia to develop?
- Oncogene activation
- Tumour suppressor gene inactivation
What are the main causes of mutations leading to cancer? (2)
- Spontaneous replication error
- Induced by carcinogens
What happens to mutated cells?
Clonal expansion.
-cells with a selective growth advantage (Darwin)
What is a common feature of most tumour cells?
Genetic instability.
-enables mutations to accumulate
What are the main tumour suppressor genes? (2)
- Gatekeepers
- Caretakers
What is the function of gatekeeper genes?
Regulate normal growth.
- negative regulators of cell cycle and proliferation
- positive regulators of apoptosis and cell differentiation
What are gatekeeper genes negative regulators of? (2)
- Cell cycle
- Proliferation
What are gatekeeper genes positive regulators of? (2)
- Apoptosis
- Cell differentiation
What is the function of caretaker genes?
Maintain genetic stability.
- repair damaged DNA
- Control mitotic accuracy
How can carcinogens affect tumour suppressor genes?
Induce molecular abnormalities»_space; decreased protein expression / inactivation.
-LOSS OF FUNCTION
What effect do mutations in caretaker genes have?
Don’t directly contribute to tumour phenotype.
-create conditions where gatekeeper mutations can arise
What does inactivation of TSGs require?
Mutations of both copies.
What normally causes the ‘1st hit’ in TSG inactivation?
Point mutation in coding sequence.
- 1 in 10 million divisions
- present in every cell in an individual with a familial cancer syndrome
What happens after a ‘1st hit’ in a TSG?
Remaining normal copy is capable of maintaining function.
-mutant version is recessive
What are the main processes that cause the ‘2nd hit’ in TSG inactivation? (3)
-Chromosomal non-disjunction
-Gene conversion
-Mitotic recombination
(-epigenetic inactivation)
Are the ‘2nd hit’ processes more or less common than point mutation?
1000x more common.
What does chromosomal non-disjunction lead to?
Aneuploidy.
-abnormal number of chromosomes
What is chromosomal recombination?
Crossing over.
- in meiosis»_space; genetic variation
- in mitosis as 2nd hit»_space; inactive TSB
Summarise the general process of TSG inactivation.
Requires mutation of both copies.
- 1st hit : normally point mutation
- 2nd hit : chromosomal non-disjunction / gene conversion / mitotic recombination
What are epigenetics?
A change in phenotype without a change in genotype.
-e.g. chemical modification
What epigentic process can cause TSG inactivation?
Promoter hypermethylation.
What do familial cancer syndromes involve?
Inheritance of a mutant copy of a caretaker / gatekeeper gene (TSG).
- ‘1st hit’
- 70-90% risk of developing cancer
FAMILIAL CANCER; what gene is involved in retinoblastoma?
RB1.