Carbohydrates Flashcards
Anthrone Test
Anthrone + Con. H2SO4
bluish-green
Dehydration > Furfural > Complex (CARBOHYDRATES)
Molisch Test
a-Naphthol + Con. H2SO4
Purple ring
Dehydration > Furfural > Complex (CARBS larger than TETROSES)
Fehling’s Test
A: Cupric Sulfate
B: Alkaline Sodium Potassium Tartrate
Yellow to Red PPT
Reduction of cupric ion (Reducing monosaccharides)
Benedict’s Test
Sodium carbonate + Sodium citrate + Copper (II) sulfate pentahydrate
Red, green, or yellow precipitate
Reduction of cupric ion (Reducing sugar)
Barfoed’s test
Cupric acetate
Red PPT
Reduction of cupric ion (Reducing monosaccharides)
Nylander’s Test
Bismuth nitrate + sodium tartrate + KOH
Black soln or PPT
Reduction of bismuth ion (Reducing sugar)
Osazone test
Phenylhydrazine + Sodium acetate
Carbonyl > phenylhydrazone > osazone (REDUCING SUGAR)
Fructose
Shape
Needle
Galactose
Thorny ball / Rhombic plate
Glucose
Needle / Broomstick
Lactose
Cotton balls / Puff
Maltose
Sunflower / Petal
Tollen’s test
AgNO3 + NH3 + NaOH
Silver mirror / Black PPT
diaminesilver(I) complex > reduces silver ion (ALDOSE)
Seliwanoff’s test
Resorcinol + Con. HCl
Red / Cherry Red PPT
Dehydration > furfural > complex (KETOSE)
Bial’s Test
Orcinol + Con. HCl + FeCl3
Bluish color
Dehydration > Furfural > complex (PENTOSE)