Cancer Treatment Flashcards
what are some drugs of alkylating agents and nitrosureas?
ifosfamide, cyclophosphamide, melphalan, chlorambucil, cisplatin, carmustine
what is the MOA of alkylating agents and nitrosureas?
addition of an alkyl group to nucleic acids/proteins/DNA
= inhibition of DNA replication = increase mutations (=secondary cancer) or cell death
any time of cell cycle
what are the main side effects of alkylating agents and nitrosureas?
teratogenic and carcinogenic
name sub classes of alkylating agents
a) Nitrogen mustards
b) Alkyl sulfonates (e.g. busulfan)
c) Triazines (e.g. dacarbazine, temozolomide)
d) Nitrosoureas (e.g. carmustine, lomustine)
e) Metal salts
what is mechlorethamine?
also known as Mustine
rarely used
short half life
very corrosive = toxic
tissue damage
nausea and vomiting side effects
what is melphalan?
oral given for myeloma - cancer of plasma cells
IV conditioning pre-transplant
increased risk of myelodysplasia with prolonged use
what is Cyclophosphamide?
A type of chemotherapy
activated in the liver, and broken down into into acrolein and etc.
what is acrolein?
bladder irritant
risk of haemarradic cystitis
what is the antidote for acrolein? and how?
MESNA
mops up the acrolein
what is bendamustine?
licenced for CLL and NHL
what are metal salts?
cisplatin, carboplatin and oxaliplatin
inhibit DNA synthesis via inter/intra- strand cross links mainly binding to GUANINE
whats cisplatin’s half life?
long half life
60 hours
what are side effects of metal salts?
nephrotoxic
neurotoxic - nause/vomiting
what are antimetabolites?
exert cytotoxic effects to natural metabolites involved in nucleic acid synthesis
The cell mistakes them for a normal metabolites, resulting in:
- inhibition of critical enzymes involved in nucleic acid
synthesis
- incorporation into the nucleic acid, leading to incorrect codes
LARGELY S-PHASE SPECIFIC
examples of anti-metabolites?
folate antagonists
pyrimidine analogues
purine analogues
ribonucleotide reductase inhibitors
MOA of 5-FU drugs
is a pyrimidine analogue that can be misincorporated into RNA and DNA in place of uracil or thymine
what is DHFR?
reduction enzyme
dihydrofolate reductase responsible for folates to be reduced to tetrahydrofolate -responsible for thymidine and purine synthesis
what is folate antagonists?
competitively inhibit DHFR= inhibit thymidine and purine synthesis
name some examples of folate antagonsists?
MTX
pemetrexed
raltitrexed
what is MTX?
inhibit dihydrofolate reductase (DHFR), leading to inhibition of DNA and RNA synthesis.
renally cleared- largely unchanged
half life- 8-10hrs may accumulate to 24-36hrs
when is folic acid prescribed w MTX?
alternate days to MTX dose
one per day or once a week
why can folic acid not be co-prescibed with MTX?
MTX 100,000x affinity for enzyme than folic acid
Folic acid may interfere with the gastrointestinal absorption of methotrexate.
what can large amounts of DHFR cause?
mtx resistance
how can mtx resistance be overcome
increase mtx dose
why is folic acid prescribed w mtx?
for treatment/rescue to healthy cells = reduce toxicity to healthy cells = decrease side effects
mtx side effects
myelosuppresion
mucositis
renal toxicity
alkalisation of urine and vigorous hydration alongside mtx administration
why do we monitor urine pH w MTX?
acidity of urine due to MTX crystallisation which can cause kidney damage
to alkalise using sodium bicarb
mtx drug interactions
aspirin/ciprofloxacin/NSAIDs = reduce tubular secretion of MTX due to renal tubular competition = increase toxicity
what is leucovorin/folinic acid?
form of THF
maintain normal cell functions - reverses MTX toxicity
when will leucovorin/folinic acid be given?
mucositis and myelsuppression
what is mucositis?
when your mouth or gut is sore and inflamed
what is pyrimidine analogues?
fraudulent nucleotides - cytosine, thymine and uracil
examples of pyrimidine analogues?
5-fluorouracil (5-FU),
gemcitabine,
capecitabine - erratic oral absorption
what is 5-FU?
a pyrimidine analogue
activated to 5-F-dUMP= inhibits thymidylate synthase =
inhibits RNA SYNTHESIS
15mins half life - therefore prolonged infusions/bolus needed
eratic oral absorption
colorectal/breast/stomach/oesophagus/head/neck carcinomas
how can we stabilise 5-F-dUMP?
co-administer folinic acid = enhances activity
what is capecitabine?
oral 5-FU drug
prodrug
conversions to 5-FU within the liver and tumour cells
breast/gastric/pancreatic cancer
is capecitabine or 5-FU better?
capecitabine better tolerated more
but experience more hand and foot syndrome
what is Gemcitabine?
Converted to gemcitabine triphosphate which is incorporated into DNA in place of deoxycytidine triphosphate.
- Given weekly.
- Licensed for pancreatic cancer, non-small cell lung cancer, bladder
cancer and breast cancer.
what is Cytarabine?
Converted to active form, ara-CTP which inhibits DNA polymerase.
- May also be directly incorporated into DNA chain, preventing replication and making it more susceptible to degradation.
- Most useful in tumours with high growth fraction, and mainly used for AML, ALL and certain lymphomas.
what is purine analogues?
Can be incorporated into the growing DNA chain in place of the natural nucleotides adenine & guanine
* Can also inhibit the enzymes involved
* May kill cells, including cancer cells
give examples of purine analogues?
6-mercaptopurine, 6-thioguanine, fludarabine
what is 6-mercaptopurine?
prodrug - oral
inhibits various metabolic reactions including purine biosynthesis
azathioprine
what is fludarabine?
The active triphosphate, 2F-ara-ATP inhibits a variety of enzymes involved in DNA synthesis
ORAL
what is an example of rinbonucleotide reductase inhibitor?
hydroxycarbamide
what is the MOA of hydroxycarbamide?
Inhibitor of ribonucleotide reductase, an enzyme essential for the generation of deoxyribonucleotides
* Prevents purines and pyrimidines synthesis= decrease DNA conc.
* May also damage DNA = inhibit DNA repair.
* Used in haematological malignancies – CML, polycythaemia,
thrombocythaemia
* Given orally as 500mg capsules – dose tailored to response
what are the antimetabolites MOA?
- folate antagonists
- inhibit the action of folates which are co-factors essential for nucleotide synthesis - pyrimidine analogues
- mimic/inhibit synthesis of cytosine, thymine & uracil thereby
interfering with DNA synthesis - purine analogues
- mimic/inhibit synthesis of adenine & guanine interfering with DNA synthesis - ribonucleotide reductase inhibitors
what are the classes of mitotic inhibitors?
vinca alkaloids -vincristine
taxanes
moa of mitotic inhibitors
act on microtubules in the nucleus = arresting metaphase = inhibit mitosis
what are examples of vinca alkaloids?
vincristine, vinblastine,
vindesine, vinorelbine
what is the MOA of vinca alkaloids?
bind to tubulin = prevent microtubule assembly
what is vincristine?
used in haematology,
indication - sarcoma and neuroblastoma
can cause neurotoxicity n mild myelosuppression
metabolised in the liver and excreted in faeces via cyto P450
what are examples of taxanes?
paclitaxel
docetaxel
abraxane
cabazitaxel
explain taxanes/ paclitaxel
indication - ovarian /breast cancer
stabilisers of microtubule
poor water solubility
risk of hypersensitivity reactions, neutropenia , neurotoxicity, alopecia, cardiotoxicity
what are Topoisomerase inhibitors?
nuclear enzymes that cause DNA strand breaks and therefore allow it to unwind during cell division.
Inhibitors stabilise the enzyme-DNA complex and prevent re-ligation, resulting in irreversible DNA strand breaks.
S/G2 phase of the cell cycle
- Topoisomerase I - causes single nick in DNA
- Topoisomerase II - cleaves both strands
what are the types of Topoisomerase inhibitors?
- Topoisomerase I - causes single nick in DNA
- Topoisomerase II - cleaves both strands
what can inhibit topoisomerase II?
etoposide