Campylobacter spp

Question 1

Characteristics

Answer 1

Gram-negative, motile, non-spore forming
Shape: slightly curved rod that appear spiral
Microaerophilic or anaerobic (3-15% oxygen and 3-5% co2)
In culture, shaped like comma or seagull
Grow on surface of solid medium

Question 2

Culturation Condition

Answer 2

Incubated for 48 hours
5% oxygen and 10% CO2

Question 3

Colonies morphology

Answer 3

Colonies are flat, droplet-like, glistening and tend to spread along the direction of the steak on moist agar. Older colonies become white to salmon-coloured.

Under transmitted light, the colonies look like intense red due to the production of cytochrome

Question 4

Campylobacter jejuni

Answer 4

Secretes toxin which destroy the mucosal epithelial
Location - target epithelial cell (small intestine)
Causes gastrointestinitis - diarrhea
Sporaradic ruminant abortion

Question 5

Laboratory diagnosis

Answer 5

Fecal samples/poultry meat/milk
Transport media : Campy-thio media (4c)
Isolation media: Colombiua blood agar
Thermophilic/microaerophilic: optimal growth at 42C
PCR
Incubation under increased CO2 and reduced 02