calibration curve Flashcards
A quantitative Benedict’s test produces a colour whose intensity depends on the concentration of reducing sugar in a solution. A colorimeter can be used to measure the intensity of this colour.
The scientist used quantitative Benedict’s tests to produce a calibration curve of colorimeter reading against concentration of maltose.
Describe how the scientist would have produced the calibration curve and used it to obtain the results in the graph.
Do not include details of how to perform a Benedict’s test in your answer.
Make/use maltose solutions of known/different concentrations
(and carry out quantitative Benedict’s test on each);
- (Use colorimeter to) measure colour/colorimeter value of each
solution and plot calibration curve/graph described;
Axes must be correct if axes mentioned, concentration on x-axis and colorimeter reading on y-axis
- Find concentration of sample from calibration curve;