C2.2 Neural Signalling Flashcards
What does the nervous system consist of?
Nervous system consists of nerve cells called neurons. In humans there are approx. 85 billion neurons. Neurons help with internal communication by transmitting nerve impulses (an electrical signal).
What are the components of the nervous systems, in hierarchical order?
What is the role of a neuron?
Neurons transmit info along nerve fibers in form of electrical impulses. Electrical impulse is not like an electrical current that flows along wires. An impulse is a momentary reversal in electrical potential difference in membrane – a change in position of charged ions between inside & outside of membrane of nerve fibres.
Annotate neuron.
What is a neuron?
Neuron is basic functional unit of nervous system. Neurons have a cell body with cytoplasm & a nucleus, as well as a long narrow outgrowth called nerve fibres along which nerve impulses travel.
What are the two types of nerve fibres?
- Dendrites (short branched nerve fibers e.g. those used to transmit impulses between neurons in one part of brain or spinal cord)
- Axons (very elongated fibers e.g. those that transmit impulses from tips of our toes/fingers to spinal cord)
What is membrane potential?
If microelectrodes are placed inside & outside any living cell, a voltage across membrane will be detected. This voltage is usually between 10 and 100 mV; it is known as membrane potential.
This potential is due to an imbalance between net charge (negative or positive) of cytoplasm & fluid outside. Cytoplasm of cells is generally electrically negative compared with fluid outside.
For this reason, membrane potential is expressed as a negative value e.g. liver cells have a potential of -40 mV.
How do neurons able to transmit information?
Nerves are able to transmit info rapidly from one part of your body to another. This info is passed along by electrical signals called nerve impulses. To record electrical activity of a nerve it is placed in an isotonic fluid bath. A reference microelectrode is placed in surrounding fluid. A recording microelectrode is inserted into cytoplasm of axon. Electrical disturbances are measured & displayed on oscilloscope. By convention outside of cell is given a value of zero, so inside has a negative relative charge ~ -70 millivolts (mV).
What is a nerve impulse?
A nerve impulse is a result of a change in concentration of sodium (Na+) & potassium (K+) ions along the cell membrane.
What are the two types of membrane potentials?
Depending on membrane potential (voltage), we can distinguish between a resting potential & an action potential.
What is resting potential?
Resting potential: this is potential difference across a nerve cell membrane when it is not stimulated (transmitting an impulse). It is at approximately -70mV.
What is action potential?
Action potential: This is sudden reversal (depolarisation) & restoration (repolarisation) of electrical potential across a plasma membrane as a nerve impulse passes along a neuron.
How is membrane potential established?
All cells have a measurable membrane potential (voltage), which is established by imbalance of ions inside & outside of a cell. Membrane potential in neurons is – 70mV.
Which three factors contribute to the establishment of negative membrane potential?
- Sodium-potassium pumps
- Leakage of ions back across membrane by simple diffusion
- Negatively charged proteins inside nerve fibre.
How does the sodium-potassium pump contribute to a negative membrane potential?
Sodium-potassium pumps in membrane transfer Na+ out of neuron & K+ into neuron at same time. This is active transport & requires ATP. Number of ions pumped is unequal – three Na+ are pumped out, two K+ ions are pumped in. This causes an imbalance of ions & conc gradients for both.
What our to steps for the mechanism involved with the sodium-potassium pump?
How does leakages of sodium and potassium ions contribute to a negative membrane potential?
Leakage of potassium & sodium ions also contributes to difference between Na+ & K+ conc gradients, increasing overall charge imbalance across membrane. Axon membrane has a higher (50x more) permeability of K+, which leaks out of cell (by diffusion) through its membrane channels faster than Na+leaks in through its Na+ protein channels.
How does negatively charged proteins inside the cell contribute to a negative membrane potential?
Due to this unequal distribution of ions, & abundance of negatively charged proteins (organic ions) inside axon fibre, membrane potential is found to be at ca. -70mV.
How is action potential measured?
Changes in membrane potential in axons during Action Potential can be measured using electrodes placed at one position along a nerve fibre. Results are displayed on an oscilloscope. Oscilloscopes are scientific instruments that are used to measure membrane potential across a neuronal membrane
Data is displayed as a graph, with time(in ms) on X axis & membrane potential (in mV) on Y axis. A typical action potential will last for roughly 3 – 5 ms & contain 4 key stages:
1. Resting potential:Before action potential occurs, neuron should be in a state of rest (approx. –70 mV)
2. Depolarisation:A rising spike corresponds to depolarisation of membrane via sodium influx (up to roughly +30 mV)
3. Repolarisation:A falling spike corresponds to repolarisation via potassium efflux (undershoots to approx. –80 mV)
4. Refractory period:Oscilloscope trace returns to level of resting potential (due to action of Na+/K+pump)
An action potential will only occur if initial depolarisation exceeds a threshold potential of approx –55 mV.
How can the cell potential be measured?
Cell potential (i.e. voltage produced by movement of ions) can be measured using microelectrodes impaled into cells. A minimal amount of stimulus is needed to fire an action potential (threshold minimum must be reached). An oscilloscope image showing changes (in mV) can be obtained.
How does can the change in potential be shown?
Change in potential difference in plasma membrane of a neurone can be shown using an oscilloscope which traces changes in voltage over time. Action potential is transported along axon fibre.
What does the action potential do to the membrane potential?
Due to an unequal distribution of ions, & abundance of negatively charged proteins inside axon fibre, membrane potential is found to be at ca. -70mV during resting potential. An action potential temporarily depolarises membrane to a positive value.
How does action potential start?
An action potential starts when Na^+ ions suddenly rush into neuron upon stimulation of voltage gated membrane channels & reverse membrane potential making it more positive.
What are the two subsequent stages that action potential is composed of?
- Depolarisation
- Repolarisation