C2.1.6 - Chromatography Flashcards
How many chemical phases are in chromatography?
2
What are the chemical phases?
Stationary and Mobile
What is the difference between the phases?
The stationary phase does not include movement but mobile does
What is the stationary phase in Paper Chromatography?
Absorbent paper
What is the mobile phase in Paper Chromatography?
Any solvent in the liquid state
What is the stationary phase in Thin-Layer Chromatography?
Thin Layer of silica or alumina powder spread over a plate of glass or plastic
What are paper and thin-layer chromatography used for?
Separating a sample into its components or soluble coloured substances
Describe the process of Thin-Layer Chromatography
Put the solvent into a chromatography tank to a depth of about 1 cm. Add a small amount of sample, taking care not to damage the powder on the plate. Let the solvent travel through the powder, and take the plate out before it reaches the top. Analyse the pattern of coloured spots, which is called a Chromatogram
What is the chromatography tank used for?
To place the substances in to start chromatography
What does the pattern produced in chromatography depend on?
How each component was distributed during each of the chemical phase
What would happen if the component forms stronger bonds with the mobile phase than with the stationary phase?
It would travel further up the plate
What would happen if the component forms stronger bonds with the stationary phase than with the mobile phase?
It would not travel very far
What is the stationary phase in Gas Chromatography?
Silica or alumina powder packed into a metal column
What is the mobile phase in Gas Chromatography?
An unreactive carrier gas
What does Gas Chromatography do?
It separates the components of a mixture and measures their amounts