c-di-GMP

Question 1

c-di-GMP is produced by which enzymes?

Answer 1

DGCs: Diguanylate cyclases

Question 2

DGCs?

Answer 2

Diguanylate cyclases

Question 3

c-di-GMP is degraded by which enzymes?

Answer 3

PDE: Phosphodiesterases

Question 4

High levels of c-di-GMP lead to which lifecycle?

Answer 4

Chronic

Question 5

c-di-GMP binding its receptor causes which lifecycle?

Answer 5

Chronic

Question 6

GTP binding its receptor causes what?

Answer 6

Higher PDE activity and lower c-di-GMP levels which causes the acute lifecycle

Question 7

What suggests a link between the Gac/Rsm and c-di-GMP pathways?

Answer 7

In retS mutants we see an increase in the c-di-GMP levels

Question 8

What cyclase in retS mutants is contributing to the increase in c-di-GMP?

Answer 8

SadC cyclase

Question 9

Acute infection is associated with?

Answer 9

Motility
Planktonic
T3SS
Production of virulence factors from QS: HCN, pyocyanin, Exotoxin A…. and from T3SS: ExoS,T,U,Y

Question 10

What leads to acute infection?

Answer 10

Quorum sensing

cAMP

Question 11

Chronic infection is associated with?

Answer 11

Sessility
T6SS
No production of virulence factors

Question 12

What leads to chronic infection?

Answer 12

Gac/Rsm… when RsmA is sequestered

High c-di-GMP levels= high DGC activity, diguanylate cyclase