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MYCOVIROLOGY MTAP 1 > BOC Study Guide (MYCOLOGY - CHAPTER REVIEW) > Flashcards

BOC Study Guide (MYCOLOGY - CHAPTER REVIEW) Flashcards

1
Q
  1. The major features by which molds are routinely categorized are:

A. macroscopic growth characteristics and microscopic morphology
B. biochemical reactions and microscopic morphology
C. macroscopic characteristics and selective media
D. specialized sexual reproductive cells and phialides

A

A. macroscopic growth characteristics and microscopic morphology

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2
Q
  1. A sputum specimen from a patient with a known Klebsiella pneumoniae infection is received in the laboratory for fungus culture. The proper procedure for handling this specimen is to:

A. reject the current specimen and request a repeat culture when the bacterial organism is no longer present
B. incubate culture tubes at room temperature in order to inhibit the bacterial organism
C. include media that have cycloheximide and chloramphenicol added to inhibit bacterial organisms and saprophytic fungi
D. perform a direct PAS stain; fi no fungal organisms are seen, reject the specimen

A

C. include media that have cycloheximide and chloramphenicol added to inhibit bacterial organisms and saprophytic fungi

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3
Q
  1. Many fungal infections are transmitted to man via inhalation of infectious structures. Which of the following is usually contracted in this manner?

A. Sporothrix schenckii
B. Trichophyton rubrum
C. Malassezia furfur
D. Histoplasma capsulatum

A

D. Histoplasma capsulatum

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4
Q
  1. Using a fluorescent microscope, a wet preparation of skin tissue reveals fluorescent septate hyphae. The smear is prepared using:

A. acridine orange
B. calcofluor white
C.Gomori methanamine silver
D.periodic acid-Schif

A

B. calcofluor white

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5
Q
  1. The formation of germ tubes presumptively identifies:

A. Candida tropicalis
B. Candida parapsilosis
C. Candida glabrata
D. Candida albicans

A

D. Candida albicans

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6
Q
  1. An HIV-positive patient begins to show signs of meningitis. A spinal fluid specimens are collected and cultured for bacteria and fungus. A budding, encapsulated yeast is recovered. Which
    organism is consistent with this information?

A. Candida glabrata
B. Cryptococcus neoformans
C.Paracoccidioides brasiliensis
D.Sporothrix schenckii

A

B. Cryptococcus neoformans

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7
Q
  1. Caffeic acid media inoculated with a yeast isolate show brown pigment production in a few hours. This is useful in the identification of:

A. Candida albicans
B. Candida glabrata
C. Saccharomyces cerevisiae
D. Cryptococcus neoformans

A

D. Cryptococcus neoformans

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8
Q
  1. The one characteristic by which an unknown Cryptococcus species can be identified as Cryptococcus neoformans is:

A. appearance of yellow colonies
B. positive urease test
C. presence of a capsule
D. positive phenol oxidase test

A

D. positive phenol oxidase test

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9
Q
  1. A Urine culture from a patient with a urinary tract infection yields a yeast with the following characteristics:

● failure to produce germ tubes
● hyphae not formed on cornmeal agar
● urease-negative
● assimilates trehalose.

The Most likely identification is:

A. Geotrichum candidum
B. Cryptococcus laurenti
C. Candida tropicalis
D. Candida glabrata

A

D. Candida glabrata

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10
Q
  1. A neonatal blood culture collected through a catheter grows a small yeast. Microscopically, or the yeast appears round at one end, with a bud-like structure on a broad base at the other end. Growth is enhanced around olive oil-saturated discs. The organism isolated is:

A. Candida tropicalis
B. Malassezia furfur
C. Candida lipolvtica
D. Cryptococcus gatti

A

B. Malassezia furfur

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11
Q
  1. The recovery of some molds and yeasts may be compromised if the isolation media contains:

A. Cycloheximide
B. Gentamicin
C. chloramphenicol
D.penicillin

A

A. Cycloheximide

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12
Q
  1. The morphological characteristics of a yeast grown in rabbit plasma are shown in the image:

The most likely identification of this yeast is:

A. Candida tropicalis
B. Candida krusei
C. Candida albicans
D. Candida glabrata

A

C. Candida albicans

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12
Q
  1. The most sensitive test for the initial diagnosis of cryptococcal disease is:

A. India ink
B. Gram stain
C. cryptococcal antigen
D. Giemsa stain

A

C. cryptococcal antigen

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12
Q
  1. Which medium would a technologist use to verify that a yeast isolated from a blood culture is pure?

A. Sabouraud dextrose agar
B. potato dextrose agar
C. cornmeal agar
D. CHROMagarTMM

A

D. CHROMagarTMM

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13
Q
  1. Which of the following procedures is recommended to confirm that an unknown mold is one of the pathogenic dimorphic fungi?

A. animal inoculation
B. conversion from yeast to mold form
C. demonstration of sexual and asexual reproduction
D. molecular testing

A

D. molecular testing

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14
Q
  1. Laboratory workers should always work under a biological safety hood when working with cultures of:

A. Streptococcus pyogenes
B. Staphylococcus aureus
C. Candida albicans
D. Coccidioides immitis

A

D. Coccidioides immitis

15
Q
  1. Structures important in the microscopic identification of Coccidioides immitis are:

A. irregular staining, barrel-shaped arthrospores
B. tuberculate, thick-walled macroconidia
C. thick-walled sporangia containing sporangiospores
D. small pyriform microconidia

A

A. irregular staining, barrel-shaped arthrospores

16
Q
  1. Which of the following is the most useful morphological feature in identifying the mycelial phase of Histoplasma capsulatum?

A. arthrospores in every other cell
B. 2-5 um microspores
C. 8-14 um tuberculate macroconidia
D. 5-7 um non septate macroconidia

A

C. 8-14 um tuberculate macroconidia

17
Q
  1. A Mold growing at 25°C exhibits delicate, septate, hyaline hyphae and many conidiophores extending at right angles from the hyphae. Oval, 2-5 um conidia are formed at the end of the conidiophores giving a flower-like appearance. The A37°C culture of this organism produces small. cigar-shaped yeast cells as shown in the images. This organism is most likely:

A. Histoplasma capsulatum
B. Sporothrix schenckii
C.Blastomyces dermatitidis
D.Acremonium falciforme

A

B. Sporothrix schenckii

18
Q
  1. Which of the following is a dimorphic fungus?

A. Blastomyces dermatitidis
B. Candida albicans
C.Cryptococcus neoformans
D.Aspergillus fumigatus

A

A. Blastomyces dermatitidis

19
Q
  1. Examination of fungal culture from a bronchial washing reveals white, cottony aerial mycelium. At ease preparation in
    lactophenol cotton blue shows the structures shown in the image:

The Most rapid test for definitive identification is:

A. MALDI-TOF mass spectrometry
B. animal inoculation
C. exoantigen test
D. slide culture

A

A. MALDI-TOF mass spectrometry

20
Q
  1. The mold shown in the image is isolated from a bone marrow culture of a patient who traveled to southeast Asia. The Isolate Produced a red pigment that diffused into the medium. The technologist should suspect

A. Blastomyces dermatitidis
B. Aspergillus niger
C. Talaromyces (formerly Penicillium) marneffei
D. Rhizopus species

A

C. Talaromyces (formerly Penicillium) marneffei

21
Q
  1. The microscopic structures that are most useful in the identification of dermatophytes are:

A. septate and branching hyphae
B. racquet and pectinate hyphae
C. chlamydospores and microconidia
D. macroconidia and microconidia

A

D. macroconidia and microconidia

22
Q
  1. The correct identification of the dermatophyte seen in this image is:

A. Epidermophyton floccosum
B. Fonsecaeapedrosoi
C. Microsporum canis
D. Trichophtontonsurans

A

A. Epidermophyton floccosum

23
Q
  1. On Day 3 of a fungal culture, a dense grayish cottony growth is observed. It fills the container and looks like “cotton candy”.
    The most likely mold isolated is a:

A. dermatophyte b
B. dimorphic mold
C. Zygomycete
D. dematiaceous mold

A

C. Zygomycete

24
Q
  1. Culture of a strand of hair, that fluoresce yellow-green when examined with a Woods lamp, produced a slow-growing, flat gray colony with a salmon-pink reverse. Microscopic examination
    demonstrated racquet hyphae, pectinate bodies, chlamydospores, and a few abortive or bizarre-shaped macroconidia. The most probable identification of this isolate is:

A. Epidermophyton floccosum
B. Microsporum canis
C. Microsporum audouinii
D. Trichophvton rubrum

A

C. Microsporum audouinii

25
Q
  1. Talaromyces (formerly Penicillium) species can be separated from Aspergillus fumigatus by:

A. production of conidia on phialids
B. optimum growth temperature
C. presence of rhizoids
D. lack of vesicle

A

D. lack of vesicle

26
Q
  1. A fungus superficially resembles Talaromyces (formerly Penicillium) species but may be differentiated because its phialides are long and tapering and bend away from the central axis, as shown in the image. The most likely identification is:

A. Exophiala
B. Acremonium
C. Cladosporium
D. Paecilomyces

A

D. Paecilomyces

27
Q
  1. An isolate from a cornea infection had the Gram stain shown in the image, and the culture results shown in the table:

*Medium
*Sabouraud dextrose
*Slide Culture

*Growth
*White & cottony at 2 days, violet
at 6 days
*White & cottony at 2 days, violet
at 6 days

The most likely organism is:
A. Acremonium
B. Aspergillus
C. Fusarium
D. Geotrichum

A

C. Fusarium

28
Q
  1. Crust from a cauliflower-like lesion on the hand exhibited brown spherical bodies 6-12 um in diameter when examined microscopically. Slow-growing black mold grew on Sabouraud dextrose agar. Microscopic examination revealed cladosporium, phialophora and fonsecaea types of sporulation. The Probable
    identification of this organism is:

A. Fonsecaea pedrosoi
B. Pseudallescheria boydii
C. Phialophora verrucosa
D. Cladosporium carrionii

A

A. Fonsecaea pedrosoi

28
Q
  1. In the USA, a common cause of eumycotic mycetoma is:

A. Pseudallescheria boydii
B. Nocardia brasiliensis
C.Coccidioides immitis
D.Aspergillus fumigatus

A

A. Pseudallescheria boydii

29
Q
  1. Which of the following scenarios presents the greatest risk for coccidioidomycosis?

A. Missouri cattle rancher
B. Wyoming rabbit rancher
C. Central Valley California migrant worker
D. pregnant woman with several house cats

A

C. Central Valley California migrant worker

30
Q
  1. Which one of the following media is most helpful in distinguishing the morphology of yeasts?

A. cornmeal agar with Tween 80
B. brain-heart infusion medium
C. potato dextrose agar
D. urea agar

A

A. cornmeal agar with Tween 80

30
Q
  1. The most likely identification of the mold shown in the images is:

A. Aspergillus niger
B. Gliocladium
C. Aspergillus fumigatus
D. Microsporum canis

A

C. Aspergillus fumigatus

31
Q
  1. Which of the following dermatophytes can be identified with its clavate or peg-shaped microconidia that resemble “birds on a wire”?

A. Microsporum canis
B. Trichophyton rubrum
C. Epidermophyton floccosum
D. Trichophyton tonsurans

A

B. Trichophyton rubrum

32
Q
  1. Which fungal organism is urease+ and phenol oxidase+?

A. Cryptococcus neoformans
B. Malassezia furfur
C. Rhodotorula
D. Trichophyton mentagrophytes

A

A. Cryptococcus neoformans

33
Q
  1. A Mold grows on SAB agar in 2 days, with “salt and pepper colonies as shown on the left image below. By day 3 the colony
    filled the petri dish. The LPCB stain of the colony is seen in the image on the right.

The fungus identification is:

A. Aspergillus
B. Cunninghamella
C. Fusarium
D. Rhizopus

A

D. Rhizopus

34
Q
  1. A dematiaceous mold grew in 4 days on Sabouraud agar, isolated from a foot wound. The image shows chaining
    macroconidia with longitudinal and horizontal septations.

The most likely identification is:

A. Alternaria
B. Curvularia
C. Paecilomyces
D. Scopulariopsis

A

A. Alternaria

35
Q
  1. This fungus is encapsulated and produces by budding. It is associated with the eucalyptus tree and is usually acquired from the environment. The organism uses glycine as a sole carbon and nitrogen source in the presence of canavanine (canavanine glycine bromothymol blue agar). The organism is:

A. Candida krusei
B. Cryptococcus gatti
C. Cryptococcus neoformans
D. Rhodotorula species

A

B. Cryptococcus gatti

MYCOVIROLOGY MTAP 1 (8 decks)

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