Blood Preparations Flashcards
anticoagulant used in hemostasis specimen collection
3.2% sodium citrate (blue top)
3.2% sodium citrate (blue top) blood-anticoagulant ratio
9:1
why EDTA is not used in hemostasis specimen collection?
EDTA irreversibly chelates calcium ions
Maintaining the blood collection tube seal minimizes CO2 diffusion, which otherwise allows the pH to rise, falsely prolonging the _____ and _____
PT and PTT
Specimens are maintained at ambient temperature, _____ to _____, never at refrigerator temperatures or on ice.
15C to 25C
Storage at 1° C to 6° C activates ______, activates platelets, and precipitates large VWF multimers.
factor VII
Specimens are never stored or transported at temperatures greater than 25° C because heat deteriorates coagulation factors __ and __
factors V and VIII
The freezer tube is labeled, sealed, and frozen. It may be stored at ___ for up to 2 weeks or at ___ for long-term storage
-20C for up to 2 weeks
-70C for long term storage
If it cannot be tested immediately, the specimen may be stored at _____ to _____ for up to ______ after thawing.
1C to 6C for up to 2 hours
(T/F)
To avoid cryoprecipitation of VWF, specimens may not be frozen and thawed more than once.
true
PRP
platelet-rich plasma
PT
prothrombin time
PTT
partial thromboplastin time
UFH
unfractioned heparin
It refers to the liquid part of an anticoagulated blood sample.
normal patient plasma (NPP)
All clotting factors are still present such as fibrinogen.
normal patient plasma (NPP)
It refers to a plasma sample (unclotted) left behind after an adsorption or simply the adherence of an antibody onto the surface of a red blood cell is done.
adsorbed plasma
_____ adsorbed plasma provides
barium sulfate
BARIUM SULFATE adsorbed plasma provides
fibrinogen
factor V, VIII, XI, XII, XIII
While the vitamin K-dependent ______ are removed
Factors II, VII, IX, X
It refers to an aged plasma sample (unclotted) deficient in Factor V & Factor VIII
aged normal plasma
Not used immediately
aged normal plasma
Used after 24 hours
aged normal plasma
It is prepared by incubating normal serum for 24 hours at 37°C.
aged normal serum
Unlike plasma, it does not contain clotting factors
aged normal serum
It is a concentrated plasma rich in platelet obtained through a higher centrifugal speed of supernatant plasma.
platelet rich plasma
Plasma with platelet count of 200,000/uL to 300,000/uL
platelet rich plasma
Relative centrifugal force (RCF) of 50 x g for 30 minutes
platelet rich plasma
PLATELET RICH PLASMA
To produce sufficient PRP, the original specimen must measure ___ to ___ mL of whole blood.
9-12mL
Are designed to test platelet rich plasma (PRP)
Light Transmittance Platelet Aggregometer (LTPA)
It is the byproduct of PRP
platelet poor plasma
It contains a very small amount of platelets.
platelet poor plasma
Plasma with platelet count of <10,000/uL
platelet poor plasma
Relative centrifugal force (RCF) of 1500 x g for 15 minutes
platelet poor plasma
Used in CLOT-BASED PLASMA COAGULATION TEST
platelet poor plasma
Plasma with platelet count of <5,000/uL
platelet free plasma
Uses DOUBLE SPIN APPROACH
platelet free plasma
Blood for whole blood platelet aggregometry, lumi-aggregometry. thromboelastography (TEG), or Rotational thromboelastometry (ROTEM) is collected with ______ and held at ___ to ___ C until testing.
3.2% sodium citrate
15C-25C
(T/F)
Chilling destroys platelet activity and is to be avoided.
true
Most specimens for whole-blood aggregometry are mixed ____ with _______ before testing
1:1 with normal saline
It is the most suitable blood preparation for detecting hypo-coagulable disorders specifically for prothrombin time (PT) test.
patient plasma
When Prothrombin time (PT) or Activated Partial thromboplastin time (APTT) is performed on test plasma deficient of any of factor II, VII, IX, X, these tests show prolonged results. When these tests are repeated after mixing Adsorbed plasma with this test plasma, PT and APTT are still prolonged i.e. not corrected. If the test plasma is deficient in factors other than factor II, VII, IX, and X, on mixing with adsorbed plasma, it will replenish those factors from adsorbed plasma, thus the tests will be said to be corrected.
adsorbed normal plasma
It is used in mixing studies; it involves combining the test plasma with normal plasma, then repeating the screening test on the mixture to assess whether the clotting time becomes normal or remains prolonged.
aged normal plasma
It is used to correct deficiency which has a normal PT, abnormal APTT and is not corrected by adsorbed plasma, Factor IX.
aged normal serum
it is used as control in coagulation studies such as APTT and PT
platelet-rich plasma
It prevents pre-analytical errors in preparing since platelets can operate as a phospholipid surface for clotting factor activation, interfering with laboratory diagnosis.
platelet-poor plasma
