Blood Clot Retraction Flashcards
When blood coagulation is complete, the clot normally undergoes retraction (serum is expressed from the clot and the clot becomes denser).
blood clot retraction
In normal clot retraction requires a normal number of functioning _____, _____, _____, and a _____ and _____.
platelets
calcium
ATP
normal fibrinogen and fibrin
An abnormal clot retraction time is found in ______ and ______.
Glanzmann’s thrombasthenia
Thrombocytopenia
the formed clot will be small and there will be increased amounts of red blood cells expressed from the clot. (RBC fall out)
dysfibrinogenemia
hypofibrinogenemia
Clot retraction may also be abnormal in ______, where the protein interferes with fibrin formation.
paraproteinemias
In ____, the formed clot will appear small and ragged, and increased RBC fall out.
DIC
the reverse occurs and the degree of clot retraction is increased.
anemia states
2 methods in blood clot retraction
single tube method
hirshboeck method / castor oil method
how many mL of blood is used in single tube method?
5mL venous blood
how many mm thick of coiled wire is used in STM?
1mm thick
incubation temperature of single tube method and for how many hour?
37C for 1 hour
STM:
Gently lift the wire and allow the attached clot to drain for _____.
2 minutes
unit used in single tube method
percentage (%) of the original volume of the whole blood placed in the tube.
reference range for STM
44-67%
Normally, a _______ serum appears within the first hour.
clear pale yellow
STM:
In 18-24 hours, the volume varies from _____ of the total amount of blood.
40-57%
STM:
When the clot is defective ad incapable of proper retraction, the volume of serum is less than _____.
less than 40%
(T/F)
Serum may appear milky after meals or in diabetes and leukemia.
true
It may be deep yellow in _____, _____, _____, or in _____.
haemolytic jaundice, leukemia, obstructive jaundice, or in carotenemia.
materials used in hirshboeck method
castor oil
WBC diluting pipette
HIRSHBOECK METHOD
Puncture sterilized finger, wipe off first drop, suck _____ of blood in a ______.
20uL
Sahli’s pipette
observations in hirshboeck method
visible dimpling or a nipple-like protrusion
clot retraction is reported delayed if dimpling happens after _____.
1 hour
clinical significance in hirshboeck method
Platelet disorders (Glanzmann’s thrombasthenia) and thrombocytopenia.
