Blood Clot Retraction

Question 1

When blood coagulation is complete, the clot normally undergoes retraction (serum is expressed from the clot and the clot becomes denser).

Answer 1

blood clot retraction

Question 2

In normal clot retraction requires a normal number of functioning _____, _____, _____, and a _____ and _____.

Answer 2

platelets
calcium
ATP
normal fibrinogen and fibrin

Question 3

An abnormal clot retraction time is found in ______ and ______.

Answer 3

Glanzmann’s thrombasthenia
Thrombocytopenia

Question 4

the formed clot will be small and there will be increased amounts of red blood cells expressed from the clot. (RBC fall out)

Answer 4

dysfibrinogenemia
hypofibrinogenemia

Question 5

Clot retraction may also be abnormal in ______, where the protein interferes with fibrin formation.

Answer 5

paraproteinemias

Question 6

In ____, the formed clot will appear small and ragged, and increased RBC fall out.

Answer 6

DIC

Question 7

the reverse occurs and the degree of clot retraction is increased.

Answer 7

anemia states

Question 8

2 methods in blood clot retraction

Answer 8

single tube method

hirshboeck method / castor oil method

Question 9

how many mL of blood is used in single tube method?

Answer 9

5mL venous blood

Question 10

how many mm thick of coiled wire is used in STM?

Answer 10

1mm thick

Question 11

incubation temperature of single tube method and for how many hour?

Answer 11

37C for 1 hour

Question 12

STM:
Gently lift the wire and allow the attached clot to drain for _____.

Answer 12

2 minutes

Question 13

unit used in single tube method

Answer 13

percentage (%) of the original volume of the whole blood placed in the tube.

Question 14

reference range for STM

Answer 14

44-67%

Question 15

Normally, a _______ serum appears within the first hour.

Answer 15

clear pale yellow

Question 16

STM:
In 18-24 hours, the volume varies from _____ of the total amount of blood.

Answer 16

40-57%

Question 17

STM:
When the clot is defective ad incapable of proper retraction, the volume of serum is less than _____.

Answer 17

less than 40%

Question 18

(T/F)
Serum may appear milky after meals or in diabetes and leukemia.

Answer 18

true

Question 19

It may be deep yellow in _____, _____, _____, or in _____.

Answer 19

haemolytic jaundice, leukemia, obstructive jaundice, or in carotenemia.

Question 20

materials used in hirshboeck method

Answer 20

castor oil
WBC diluting pipette

Question 21

HIRSHBOECK METHOD
Puncture sterilized finger, wipe off first drop, suck _____ of blood in a ______.

Answer 21

20uL
Sahli’s pipette

Question 22

observations in hirshboeck method

Answer 22

visible dimpling or a nipple-like protrusion

Question 23

clot retraction is reported delayed if dimpling happens after _____.

Answer 23

1 hour

Question 24

clinical significance in hirshboeck method

Answer 24

Platelet disorders (Glanzmann’s thrombasthenia) and thrombocytopenia.