Blood Groups & Donation Flashcards
Minimum Hb criteria for men to donate blood?
135g/L
Minimum Hb criteria for women to donate blood?
125g/L
Minimum weight for blood donors?
50kg
Blood donation samples undergo microbiological testing for what 6 diseases?
HIV, Hep B, Hep C, Hep E, HTLF, Syphilis
At what temp and for how long is FFP stored for?
stored at 4 degrees for 35 days
At what temp and for how long is FFP stored for?
stored at -30 degrees for 3 years
At what temp and for how long is FFP stored for?
stored at 22 degrees for 7 days with agitation (shaking)
What blood components are available for transfusion from lab? (4)
red cells,
FFP,
platelets,
cryoprecipitate (source of fibrinogen)
Give 2 examples of blood products available for transfusion from lab
Anti-D immunoglobulin,
prothrombin complex concentrate (reverse warfarin)
Give 3 examples of blood products available from pharmacy?
IV immunoglobulin,
human albumin,
specific Ig e.g. zoster Ig
ABO system coded for chromosome on what chromosome?
Chromosome
A and B genes code for what?
for transferases which modify precursor called H substance on red cell membrane
What does it mean that O is silent in ABO?
O means you lack A or B so both A and B groups so you don’t express A or B antigens on your RBCs
A and B are dominant over O and A is dominant over B. T/F?
FAlse!! A and B are dominant over O and A and B are co-dominant
What is most common ABO group from most to least and their population frequency percentage?
O - 47%,
A - 42%,
B - 8%,
AB - 3%
What is difference between phenotype and genotype with blood groups?
Phenotype is which antigens are detected whereas genotype which genes are present
Genotype of phenotype Group O?
OO
Genotypes of phenotype Group A?
AA or AO
Genotypes of phenotype Group B?
BB or BO
Genotype of phenotype Group AB?
AB
What does Landsteiner’s Law state?
When an individual lacks the A or B antigen, the corresponding antibody is produced in their plasma
Blood group A express what antibodies in their plasma?
anti-B
Blood group B express what antibodies in their plasma?
anti-A
Blood group O express what antibodies in their plasma?
anti-A and anti-B
Blood group AB express what antibodies in their plasma?
neither
Blood group O can receive what blood?
only other O
Blood group A can receive what blood?
only A and O
Blood group B can receive what blood?
only B and O
Blood group AB can receive what blood?
all types
85% of population are RhD positive/negative?
positive
RhD + genotype?
DD or Dd
RhD - genotype?
dd
Anti-D antibody can cause what 2 types of reactions?
transfusion reactions,
haemolytic disease of the foetus/newborn
RhD positive blood should NEVER be given to RhD negative patients. T/F?
True
Although less immunogenic than ABO and Rh systems, when might other blood group systems lead to transfusion reaction?
if sensitised to exposure to foreign antigens by pregnancy/transfusion, repeat exposure can cause transfusion reaction
What is antisera and what is regent red cells? How do they interact?
antisera: reagant with known antibody specificity used to identify antigens present on red cells,
reagant red cells: red cells with known antigen specificity to identify antibodies in plasma
together there is agglutination of red cells
How is blood ABO/D grouping tested?
test patient’s red cells with anti-A, anti-B and anti-D antisera
