Biotechnology in Medicine and Agriculture

Question 1

Cloning

Answer 1

Is the production of identical copies of DNA, cells, or

Question 2

Gene cloning

Answer 2

Is the production of many identical copies of the same
gene.

Humans can be treated with gene therapy.

Question 3

Recombinant

Answer 3

DNA (rDNA) contains DNA from two or more different sources.

A vector
• It introduces rDNA into host cell.
• Plasmids (small accessory rings of DNA from bacteria) are common vectors.

Two enzymes are required to introduce foreign DNA into vector DNA.
• A restriction enzyme cleaves DNA.
• A DNA ligase enzyme seals DNA into an opening created by the restriction enzyme.

Question 4

What do Restriction Enzyme

Answer 4

Cut DNA at specific points and makes sticky ends which allows foreign DNA

Question 5

Polymerase Chain Reaction (PCR)

Answer 5

It amplifies a targeted sequence of DNA

Question 6

What is Short tandem repeat profiling (STR)

Answer 6

Technique that is used to analyze DNA fragment lengths