Biotechnology 1 Lecture 4

Question 1

how does the testing activity spectrum owrk

Answer 1

streak antibiotic producer on one end, streak diff bacterial strains perpendicular to antibiotic, observe inhibition zones away from antibiotic

Question 2

explain cross streak method

Answer 2

spread producer on a plate and let incubate. put bac strains around producer- observe if any zones of inhibition

Question 3

write a note on penicillins

Answer 3

these are B lactam antibiotics. there are two types: natural and biosynthetic, or semisynthetic penicillins which have a broad spectrum of activity

Question 4

when does penicillin production begin, and how does glucose affect production

Answer 4

production only starts after near exhaustion of carbon source. high glucose represses penicillin production