BIOTECH PROCESSES Flashcards
How many types of biotech are there?
Conventional/traditional
Modern
How many DNA’s are there?
Chromosomal DNA
Plasmid DNA
Molecular scissors are c/d?
Restriction enzymes
Molecular glue?
DNA ligase
Principles of Biotechnology?
- Genetic engineering
- Biochemical engineering
1st Recombinant DNA constructed in? By? How?
DIAGRAM MARATHON PDF SE DEKH LIYO!
1972
Stanley Cohen and Herbert Boyer
linked-antibiotic-resistant-gene-with-native-plasmid-of-Salmonella typhimurium
E coli has?
Restriction endonuclease
Methylase
Restriction enzymes belong ?
the enzymes break?
RE obtained from?
Today we have more than _____ obtained from _____ of bacteria
category-enzymes-Nucleases
phosphodiester bond
prokar
900 , 230 strains
Types of RE?
EXONUCLEASE
ENDONUCLEASE
1ST RE? isolated and characterised??
Hind-II
5 years later
Palindromic sequence of EcoRI
6 base pair - GAATTC
Endonuclease makes how many cuts?
2
blunt/flush ends
sticky / over handing ends
agarose gel obtained from?
sea weed (red algae)
Dna is loaded on? acc to?
-ve cathode side in wells
molecular weight
Denaturation happen at?
90-95 degree celcius
In annealing, where is oligonucleotide primer attached? temp?
3 prime OH end
50-55 degree celcius
extension temp? what is used? obtained from? if 30 cycles are done..then how many ??
72 degree celsius
Taq-polymerase
Thermus aquaticus
thermostable enzyme
1 billion dna fragments
selectable marker helps in distinguishing?
Recombinant DNA
Non- Recombinant DNA
Transformant
Non-transformant
Agrobacterium tumifaciens is a pathogen of ? is able to? why?
dicot plants
deliver a piece of DNA k/as T-DNA
transform normal plant cell into a tumor cell
What are the types of bioreactors?
- Simple stirred bioreactor
- Sparged stirred bioreactor
