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exam > biotech > Flashcards

biotech Flashcards

1
Q

series of proteins built into
inner mitochondrial membrane

A

Electron Transport Chain (ETC)

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2
Q

where does ETC take place in mitochondria?

A

cristae (inner membrane)

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3
Q

ETC

A

transport of electrons down ETC linked to
pumping of H+
to create H+ gradient

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4
Q

(True or false) yields ~32 ATP from 1 glucose and is anaerobic respiration.

A

false, yields ~36 ATP, aerobic respiration

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5
Q

highly folded cristae, enzymes & transport
proteins

A

inner membrane

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6
Q

fluid-filled space
between membranes

A

intermembrane space

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7
Q

other name for Complex I?

A

NADH
dehydrogenase

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8
Q

other name for Complex III?

A

cytochrome
bc complex

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9
Q

other name for Complex IV?

A

cytochrome c
oxidase complex

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10
Q

What powers the proton (H+) pumps

A

ATP

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11
Q

what “pulls” the
electrons down the ETC?

A

O2

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12
Q

The diffusion of ions across a membrane

A

Chemiosmosis

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13
Q

links the Electron
Transport Chain
to ATP synthesis

A

Chemiosmosis

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14
Q

Proposed chemiosmotic hypothesis, (revolutionary idea at the time)

A

Peter Mitchell

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15
Q

Where did the glucose come from?

A

Food

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16
Q

Where did the O2 come from?

A

Air

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17
Q

Where did the H2O come from?

A

ETC

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18
Q

What is the last acceptor of etc?

A

oxygen

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19
Q

a solid, liquid or semi-solid
designed to support the growth of microorganisms or cells.

A

A growth medium or culture medium

20
Q

a general purpose, nutrient medium used for the cultivation of microbes

A

Nutrient Agar

21
Q

principal source of organic nitrogen for the growing bacteria.

A

0.5% Peptone

22
Q

water-soluble substances which aid in bacterial growth, such
as vitamins, carbohydrates, organic nitrogen compounds and salts

A

0.3% beef extract

23
Q

solidifying agent.

A

1.5% agar

24
Q

maintains a salt concentration in the medium that is similar to the
cytoplasm of the microorganisms.

A

0.5% NaCl

25
Q

Water is essential for the growth of and reproduction of microorganisms and also provides the medium through which various nutrients can be
transported.

A

Distilled water

26
Q

pH is adjusted to neutral ?

A

(7.4) at 25 °C.

27
Q

Uses of Nutrient Agar

A

for isolation and purification of culture

28
Q

contain all the elements that
most bacteria need for growth and are not
selective, so they are used for the general
cultivation and maintenance of bacteria kept in
laboratory culture collections

A

Culture media

29
Q

those that contain the
minimum nutrients possible for colony growth,
generally without the presence of amino acids,
and are often used by microbiologists and
geneticists to grow “wild-type”
microorganisms

A

Minimal Media

30
Q

Types of media

A

Culture, Minimal, Differential, selective, transport, enriched media

31
Q

used for the growth of only selected microorganisms

A

Selective media

32
Q

true or false. MacConkey agar is for Gram-negative bacteria and is differential for lactose fermentation

A

true

33
Q

distinguish one microorganism type from
another growing on the same medium

A

differential media

34
Q

contains bovine heart blood that
becomes transparent

A

blood agar

35
Q

used in the isolation of anaerobes must be free of molecular
oxygen

A

Transport Media

36
Q

nutrients required to support the growth of
a wide variety of organisms

A

enriched media

37
Q

enriched with heat-treated blood (40–
45 °C), which turns brown and gives the medium the color for which it
is named

A

chocolate agar

38
Q

TRUE OR FALSE. Steps in weighing using an analytical balance.
ZERO THE SCALE BY PRESSING THE TARE BUTTON. THE
SCALE READING SHOULD BE “0”.
* PLACE THE WEIGHING BOAT OR DISH ON THE SCALE.
THERE IS NO NEED TO RECORD THIS VALUE.
* PRESS THE “TARE” BUTTON ON THE SCALE. THE BALANCE
READING SHOULD BE “0”.
* ADD THE SAMPLE TO THE CONTAINER. THE
VALUE GIVEN IS THE MASS OF YOUR SAMPLE. RECORD IT.

A

true

39
Q

isolating specific bacteria from a
sample containing a mixture of microorganisms.
The technique essentially dilutes the number of
organisms and reduces their density.

A

streak plate technique

40
Q

a visible cluster of bacteria

A

colony

41
Q

inoculating the top half of the plate,
rotating it 180 degrees, and inoculating the
other half of the plate without sterilizing the
loop or dragging bacteria from the previous
section

A

continuous streaking

42
Q

isolating individual cells by preparing areas
of dilution (isolation)

A

Quadrant streaking

43
Q

divided into three quadrants by drawing
the letter ‘T’.

A

T-streaking

44
Q

True or false. Isolated colonies are produced but large fuzzy white colonies indicate contamination. This shows great streaking.

A

false

45
Q

results from leaving the plate open too long or not shielding properly with the lid

A

contamination of a streak plate

exam (9 decks)

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