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Biochem > Biomolecule Identifying Tests > Flashcards

Biomolecule Identifying Tests Flashcards

1
Q

What does the Sudan test look for?

A

Lipids

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2
Q

What result does dark red on the Sudan test indicate?

A

Negative for lipids

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3
Q

What a result of two layers of light red and red indicate on the Sudan test?

A

Positive for lipids

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4
Q

What is the Biuret test used to detect?

A

Proteins

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5
Q

What does purple on the Biuret test indicate?

A

Positive for proteins

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6
Q

What does blue on the Biuret test indicate?

A

Negative for proteins

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7
Q

What is the Bradford protein assay?

A

Quantitatively determines how much protein is present with Coomassie Brilliant Blue dye

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8
Q

What is the Ninhydrin test used to detect?

A

Amino acids based on presence of carboxyl groups of proteins peptides

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9
Q

What does purple on the Ninhydrin test indicate?

A

Positive for amino acids

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10
Q

What does yellow on the Ninhydrin test indicate?

A

Positive for proline

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11
Q

What does blue on the ninhydrin test indicate?

A

Negative

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12
Q

What is the Benedict’s test used to detect?

A

Reducing sugars (all monosaccharides bearing aldehyde groups in open form)

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13
Q

What does blue on the Benedict’s test indicate?

A

0% reducing sugars

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14
Q

What does Lugol’s iodine solution test indicate

A

Starch

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15
Q

What color is a positive Lugol’s solution test

A

Dark blue/black

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16
Q

What does a dark blue/black lugol’s solution test indicate

A

Positive for starch

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17
Q

What color is a negative Lugol’s solution test

A

Reddish orange

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18
Q

What does a reddish orange lugol’s solution test indicate

A

Negative for starch

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19
Q

What color is a negative sudan’s test

A

Dark red

20
Q

What color is a positive sudan’s test

A

2 layers of light red and red

21
Q

What color is a positive Biuret’s test

A

Purple

22
Q

What color is a negative Biuret’s test

A

Blue

23
Q

What color is a positive Ninhydrin’s test

A

Purple

24
Q

What color is a negative Ninhydrin’s test

A

Blue

25
Q

What color is positive for proline Ninhydrin’s test

A

Yellow

26
Q

What color is a negative Benedict’s test

A

Blue

27
Q

What colors are the positive Benedict’s test and their percentages

A

Teal (0-0.5%), Green (0.6-1%), light green (1.1-1.5%), Yellow (1.6-2.5%), orange (2.6-3.5%), red (3.6-4%), brown (>4%)

28
Q

What is the difference between ketones and aldehydes

A

Aldehyde (carbonyl group at end) is easily oxidized, ketone (carbonyl group in carbon chain) have no particular H atom, resistant to oxidation

29
Q

What does no change in colorless solution on Tollens silver mirror test indicate

A

Presence of ketone, no aldehyde

30
Q

What does the production of a grey precipitate of silver indicate on Tollens silver mirror test

A

Presence of aldehyde

31
Q

What result indicates ketone presence/no aldehyde for tollens silver mirror test

A

No change in colorless solution

32
Q

What result indicates aldehyde presence for tollens silver mirror test

A

Grey precipitate in silver solution

33
Q

What does no change in blue solution indicate for Fehling’s test

A

Ketone presence, no aldehyde

34
Q

What does dark red precipitate of red copper I oxide on fehling’s test indicate

A

Presence of aldehyde

35
Q

What precipitate is produced in the presence of aldehyde for Fehling’s test

A

Dark red of copper I oxide

36
Q

What result indicates aldehyde presencefor fehlings

A

Dark red precipitate of copper I oxide

37
Q

What result indicates ketone/no aldehyde presence

A

No change in blue solution

38
Q

What test is used to test for presence of nucleic acids

A

Diphenylamine test

39
Q

What does blue on diphenylamine test indicate

A

DNA presence

40
Q

What does green on diphenylamine test indicate

A

RNA presence

41
Q

What does colorless on diphenylamine test indicate

A

Negative for nucleic acids

42
Q

What result indicates DNA presence on diphenylamine test

A

blue

43
Q

What result indicates RNA presence on diphenylamine test

A

green

44
Q

What result indicates no nucleic acids on diphenylamine test

A

Colorless

45
Q

What does the diphenylamine test indicate

A

Presence of nucleic acids

Biochem (5 decks)

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