Biomembrane Flashcards

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1
Q

what is a lipid bilayer?

A

provides the basic structure for all cell membranes, double-layered sheets with hydrophobic tails sandwiched between the hydrophilic heads

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2
Q

what is amphiphilic?

A

have a hydrophobic and hydrophilic end

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3
Q

polar molecules dissolve _____ in water

A

well

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4
Q

what happens when you add nonpolar molecules in water?

A

it cannot form favorable interactions with water so it forces adjacent water molecules to reorganize into ice-like cage structures, increasing free energy

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5
Q

what is a micelle?

A

cone-shaped molecules with their hydrophobic tail inwards

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6
Q

why does a phospholipid bilayer close to form a sealed compartment?

A

edges of the bilayer are exposed to water so it curls up into itself to avoid water exposure which is energetically unfavorable

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7
Q

what is the difference between cytosolic and exoplasmic?

A

exoplasmic is outside of the cell and cytosolic is inside the cell

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8
Q

how does endocytosis/exocytosis work?

A

lipid bilayer pinches in and encloses the exoplasmic/endoplasmic portion within the lumen

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9
Q

what are the 3 main categories of lipids?

A

phosphoglycerides, sphingolipids, sterols

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10
Q

what are phosphoglycerides?

A

form lipid bilayer, most abundant, two fatty acid tails, phosphate group, head group, glycerol

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11
Q

what are sphingolipids?

A

forms lipid bilayer, long fatty acid chain attached to sphingosine via amide linkage, different head group,

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12
Q

what are the 4 major lipids in mammalian plasma membrane?

A

phosphatidylcholine, phosphatidylserine, phosphatidylethanolamine, phosphatidylinositol

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13
Q

what are sterols?

A

4 ring hydrocarbon, abundant in mammalian cells but not in prokaryotes, -OH group interacts with water. usually embedded in membrane to give structure but cant form full lipid membrane

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14
Q

how are sterols represented in animals?

A

cholesterol

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15
Q

how are sterols represented in fungi?

A

ergoterol

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16
Q

how are sterols represented in plants?

A

stigmasterol

17
Q

why cant sterols form a lipid bilayer on their own?

A

too hydrophobic

18
Q

can lipids move laterally?

A

yes

19
Q

how do you test to see if lipids move laterally?

A

FRAP (fluorescence recovery after photobleaching)

20
Q

what is FRAP?

A

you label a certain section with fluorescence then bleach it. wait and see if it stays bleached or not. used to see for lipid movement

21
Q

what is photoactivation?

A

add fluorescence to an area and see if it speads

22
Q

when do lipids flip-flop?

A

almost never. usually laterally or rotation

23
Q

what is thicker unsaturated double bonds or saturated bonds?

A

saturated bonds

24
Q

what are lipid rafts?

A

microdomain of less-fluid lipids distributed among more fluid lipids, lipids not evenly distributed, also contains receptor proteins and signaling proteins

25
Q

how thick is sphingomyelin?

A

super thick, the thickest

26
Q

cone shaped lipids ____ the membrane

A

curve

27
Q

do organelles have different lipid composition?

A

yes

28
Q

what are polarized cells?

A

cells that have a different lipid composition on both sides and interact with different things

29
Q

exoplasmic contains mostly

A

less fluid lipids

30
Q

cytoplasmic contains mostly

A

more fluids

31
Q

phosphorykated PI plays a role in ____

A

signalling

32
Q

PS is ____ in the cytosolic leaflet

A

abundant, will only go outside when cell is dying

33
Q

what are transmembrane proteins?

A

spans the lipid bilayer, extracelluar, transmembrane, cytosolic domain

34
Q

what is GPI anchor?

A

sugar, glycine, phospjoethanolamine