biomed Flashcards
what is dna
Blueprint for our physical traits. Also a double stranded, helical nucleic acid molecule capable
of replicating and determining the inherited structure of a cell’s proteins.
How do scientists isolate DNA in order to study it?
DNA extraction. You extract DNA from the other parts of the cell, the buffer/detergent (soap)
breaks down the cell membrane, alcohol precipitates the DNA (separates the DNA and allows
it to float to the surface)
How does DNA differ from person to person?
The only difference between two people’s DNA is the sequence of the base pairs
How can tools of molecularbiology be used to compare the DNA of two individuals?
The restriction enzymes and gel electrophoresis can be used to match or compare DNA. PCR
can be used to make more copies of DNA if there isn’t enough to examine
what are restriction enzymes
Enzymes that cut DNA into fragments at specific patterns (ex GC| CG)
how do you read restriction enzymes
left to right
which strand do you use to read restriction enzymes
the top one
What are restriction fragment length polymorphisms(RFLPs)?
Different sized fragments of DNA produced from treatment with restriction Enzymes. Size is
determined by how many base pairs the fragment is made up of.
can people make the same RFLP?
Since every one’s DNA is
different, no two people will produce the same RFLPs
What is gel electrophoresis and how can the results ofthis technique be interpreted?
Gel electrophoresisis amolecular analysis technique used to identify the presence of genes
and to identify unknown DNA samples. Results are interpreted by comparing the known and
unknown lanes.
adenine
A component of nucleic acids, energy-‐carrying molecules such as ATP, and certain coenzymes. Chemically, it is a purine base.
chromosome
any of the usually linear bodies in the cell nucleus that contains the genetic material
cytosine
A component of nucleic acids that carries hereditary information in DNA and RNA in cells. Chemically, it is a pyrimidine base.
dna
A double-‐stranded, helical nucleicacid molecule capable of replicating and
determining the inherited structure of a cell’s proteins.
gel electrophoresis
The separation of nucleic acids or proteins, on the basis of their size and electrical charge, by measuring their rate of movement through an electrical field in a gel.
gene
A discrete unit of hereditary information consisting of a specific nucleotide sequence in DNA (or RNA, in some viruses).
guanine
A component of nucleic acids that carries hereditary information in DNA and RNA in cells. Chemically, it is a purine base.
helix
something in spiral form
nucleotide
A building block of DNA, consisting of a five-‐carbon sugar covalently bonded to a nitrogenous base and a phosphate group.
restriction enzyme
a degradative enzyme that recognizes specific nucleotide sequences and cuts up DNA
restriction fragment length polymorphisms (RFLP’s)
Differences in DNA sequence on homologous chromosomes that can result in different patterns of restriction fragment lengths(DNA segments resulting from
treatment with restriction enzymes).
thyamine
A component of nucleic acid that carries hereditary information in DNA in cells.
Chemically, it is a pyrimidine base.