Biomarkers of Immune Cells Flashcards
1
Q
Markers for tumor-associated macrophages (TAMs)?
A
CD45+ CD11b+ Ly6G− Ly6C− F4/80+
2
Q
Markers for activated cytotoxic T-cells?
A
IFN-γ+
GzmB+
3
Q
Where is CD31 expressed?
A
CD31 (aka PECAM-1) is found on:
- platelets
- monocytes
- neutrophils
- some types of T-cells
- makes up a large portion of endothelial cell intercellular junctions.
4
Q
What is the effect of using anti-CSF1R antibody on TAM populations in pre-clinical studies?
A
Causes TAM depletion
5
Q
CD44
A
Marker of effector-memory T-cells
6
Q
ki-67
A
cell-cycle marker - present in recently dividing or cycling cells
7
Q
Annexin A5 (or annexin V)
A
- cellular protein in the annexin group.
- In flow cytometry, Annexin V is commonly used to detect apoptotic cells by its ability to bind to phosphatidylserine, a marker of apoptosis when it is on the outer leaflet of the plasma membrane.
8
Q
CD154 aka CD40 ligand or CD40L
A
- protein that is primarily expressed on activated T cells
- member of the TNF superfamily of molecules.
- binds to CD40 on APC –> many effects depending on the target cell type.
9
Q
L-selectin aka CD62L
A
- Central memory T-lymphocytes, which have encountered antigen, express L-selectin to localize in secondary lymphoid organs. Here they reside ready to proliferate upon re-encountering antigen.
- Effector memory T-lymphocytes do not express L-selectin, as they circulate in the periphery and have immediate effector functions upon encountering antigen.
