Biology Flashcards
Semi conservative replication
2 DNA strands unzip and each are a template for complementary nucleotides to form new strands ( 2 identical chromosomes)
Topoisomerases
Relax supercoiling
Helicase
Disrupts H-bonds between bases which unzips DNA
DNA primase
Forms short RNA primer which initiates binding of DNA polymerase to parent strands
DNA polymerase
Binds to parent strand and travels 5’ to 3’
Parent strands are antiparallel which means?
DNA polymerases want to go in opposite directions
Leading strand is synthesized __________?
Continuously
Lagging strand is synthesized in ___________?
Okazaki fragments ( 200-2000 base pairs separated by 50 base pair gaps )
Ligase fills gaps between __________?
Okazaki fragments
_______________ replaces RNA primer with DNA nucleotides
Another DNA polymerase
DNA
Single origin of replication and 2 replication forks move in opposite directions around chromosome
Eukaryotic DNA
Multiple linear chromosomes sopercoiled around histone proteins, multiple origins of replication
Telomeres
Numerous at ends of eukaryotic chromosomes and prevent damage to coding regions
________ lost from ends of chromosomes with each replication
1
When the last telomere is lost, more replication causes chromosome damage which is associated with?
Aging
Centromere
Area of a eukaryotic chromosome with repeating bases
__________ remain connected at the centromere until disjunction in mitosis or meiosis
2 sister chromatids
Okazaki fragments are much shorter in eukaryotes because of differences in ______?
Polymerases
Transcription ______ to ______?
DNA to RNA
Translation ______ to __________?
RNA to amino acids sequence of proteins
______ of genome transcribed
Only a fraction
Transcribed section of genome?
Gene
Adjacent genes expressed together? (name)
Operon
3 steps of Transcription?
Initiation, Elongation, and Termination