Biology Flashcards
What is biology?
The study of living things
What is spontaneous generation?
It was believed that living life originated from the non-living/dead via spontaneous generation
This idea was made popular by Aristotle
Who was Francesco Redi?
First to conduct a controlled experiment to test the concept of spontaneous generation
He suspected that the maggots that appeared on rotting meat did not actually arise from the meat itself
He hypothesized that maggot hatched from the objects dropped by the flies
What were some aspects of Redi’s Experiment?
Control Group
- no cloth covering
- flies go in and out
Experimental Group
- cloth covering
- flies were able to go in and out
Results
- control: meat had maggots
- experimental: no maggots
Conclusion
- maggots found in rotting meat were not due to spontaneous generation
What is Active Principle?
The discovery of microorganisms by the use of microscopes re-opened the discussion about the origin of life
Many people believed that the air has an ‘active principle’ that helps with the generation of these microorganisms
Louis Pasteur hypothesized that this ‘active principle’ in the air was an organism
Who was Pasteur?
Devised an experiment with a unique S neck flask
Broth was placed in the flask and then boiled to kill all the microorganisms
As each flask cooled, fresh air was drawn in and moisture condensed in the curve of the neck
Pasteur predicted that air entering the neck of the flask would contain microorganisms but that they would settle by gravity in the neck and never reach the broth
What is biogenesis?
The theory that living organisms could arise only from other living things
German physician Rudolf Virchow suggested the theory in 1858
Pasteur’s experiments provided supporting evidence for biogenesis
Who was Robert Hooke?
Observed cork from the bark of an oak tree under a microscope
It looked as it the cork was made up of hundred of tiny rooms
They reminded him of monks tiny rooms or cells in a monastery, he named the structures cells
Who was Antony Van Leeuwenhoek?
Became the first person to describe single-celled microorganisms
Referred to them as ‘animalcules’
Who were Matthias Schlediden and Theordor Schwann?
Worked together by comparing results and came to an important conclusion
Schleiden noted that all plants are composed of cells
Schwann concluded that animal tissues contained cells
Who was Rudolf Virchow?
Showed in experiments that bone cells could develop from cartilage cells
Made microscope observations of divining cells from multicellular organisms
What is cell theory?
All organisms are composed of one or more cells
The cells is the basic unit of structure and organization in organisms
All cells come from pre-existing cells
What are simple compound light microscopes?
Use light to pass through a specimen and 2 or more lenses to create an image
What are the pros and cons of simple compound light microscopes?
Pros:
- inexpensive
- small in size
- transportable
Cons:
- low magnifying power
- creates 2D images
What are electron microscopes?
Specimens are illuminated with a beam of electrons instead of a beam of light
Magnifies images up to 1.2 million times
What are the pros and cons of electron microscopes?
Pros:
- enhanced image quality
- greater magnifying power
- high resolution
Cons:
- costly
- size
- stationary
Staining Cells
Scientists stain cells so they can see various structures/organelles with the cell
This allows for greater clarity
Different chemicals attach to different structures to accentuate them when viewed under a microscope
What is a Scanning Electron Microscope (SEM)?
The SEM sweeps a beam of electrons over an object to create a three-dimensional image
SEM can reach a magnification of 300 000x
What is a Transmission Electron Microscope (TEM)?
Canadian scientists helped improve the design of the microscope
A beam of electrons is transmitted through a specimen to produce a 2D image magnified 10 000x to 100 000x
What is a Confocal Laser Scanning Microscope (CLSM)?
A laser beam is first directed at one plane, then another, and so on. This process creates a series of two dimensional images
The CLSM produces optical ‘slices’ of a three-dimensional object
What is a Scanning Tunnelling Microscope?
Allows scientists to see at the atomic level
Greater magnifying power than electron microscopes
How do you calculate Field of View?
HP FOV LP Mag
———- = ————
LP FOV HP Mag
You multiply the LP FOV by the LP Mag then divide that number by the HP Mag
What is the formula for actual size?
AS = FOV
———
Fit #
Cell Divison
Discovered as early as 1842
Has advanced from the observation of cells splitting in two to the description of the movement and activity of the chromosomes under the cell during cell divison